Brassinosteroid Biosynthetic Gene SlCYP90B3 Alleviates Chilling Injury of Tomato (Solanum lycopersicum) Fruits during Cold Storage.
Brassinosteroid Biosynthetic Gene SlCYP90B3 Alleviates Chilling Injury of Tomato (Solanum lycopersicum) Fruits during Cold Storage.
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DOI：
10.3390/antiox11010115
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发表时间：
2022-01-05
期刊：
影响因子：
--
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Hu S;Wang T;Shao Z;Meng F;Chen H;Wang Q;Zheng J;Liu L
番茄在冷藏过程中易发生冷害。在本研究中，我们发现低温促进番茄果实中油菜素类固醇（BR）生物合成基因的表达。BR生物合成关键基因SlCYP 90 B3（SlCYP 90 B3-OE）的过表达可减轻低温伤害，降低电导率和丙二醛含量。与野生型相比，S1 CYP 90 B3-OE番茄果实抗氧化酶活性（APX、CAT、POD、SOD）显著提高，膜脂分解酶活性（LOX、PLD）显著降低。此外，冷响应系统组分SlCBF 1的表达水平在SlCYP 90 B3-OE果实中比在野生型果实中更高。这些结果表明，SlCYP 90 B3可能通过调节抗氧化酶系统和SlCBF 1的表达，参与番茄果实耐冷性的调节。
Tomato is susceptible to chilling injury during cold storage. In this study, we found that low temperature promoted the expression of brassinosteroid (BR) biosynthetic genes in tomato fruits. The overexpression of SlCYP90B3 (SlCYP90B3-OE), a key BR biosynthetic gene, alleviated the chilling injury with decreased electrical conductivity and malondialdehyde. In SlCYP90B3-OE tomato fruits, the activities of antioxidant enzymes, including ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), were markedly increased, while the activity of membranous lipolytic enzymes, lipoxygenase (LOX), and phospholipase D (PLD), were significantly decreased when compared with the wild-type in response to cold storage. Furthermore, the expression level of the cold-response-system component, SlCBF1, was higher in SlCYP90B3-OE fruits than in the wild-type fruits. These results indicated that SlCYP90B3 might be involved in the chilling tolerance of tomato fruits during cold storage, possibly by regulating the antioxidant enzyme system and SlCBF1 expression.
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