Proteomic analysis of pollination-induced corolla senescence in petunia.
Proteomic analysis of pollination-induced corolla senescence in petunia.
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DOI：
10.1093/jxb/erp373
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发表时间：
2010-02
影响因子：
6.9
通讯作者：
Jones ML
中科院分区：
文献类型：
--
作者：
Bai S;Willard B;Chapin LJ;Kinter MT;Francis DM;Stead AD;Jones ML
衰老代表花瓣发育的最后阶段，在此期间，大分子和细胞器被降解，营养物质被回收到发育中的组织。为了更好地了解花瓣衰老的转录后调控机制，采用蛋白质组学方法分析了矮牵牛"Mitchell二倍体“花冠衰老过程中的蛋白质变化。从未授粉的矮牵牛花冠中提取总可溶性蛋白质，分别在开花后0，24，48和72 h以及授粉后24，48和72 h提取。使用二维凝胶电泳（2-DE）来鉴定在非衰老（未授粉）和衰老（授粉）花冠中差异表达的蛋白质，并且使用图像分析来确定哪些蛋白质通过实验确定的2.1倍截止值（P <0.05）上调或下调。选择133个差异表达蛋白点进行测序。采用液相色谱-串联质谱法（LC-MS/MS）确定这些蛋白的同一性。检索翻译的EST数据库和NCBI非冗余蛋白质数据库，可以将推定的鉴定分配给大于90%的这些蛋白质。许多衰老上调的蛋白质参与防御和应激反应或大分子催化。一些蛋白质，没有以前的特点，在花衰老过程中，被确定，包括番茄脱落酸胁迫成熟蛋白4（ASR 4）的直系同源物。基因表达模式并不总是与蛋白质表达相关，证实了蛋白质组学和基因组学方法将需要获得花瓣衰老的调控的详细了解。
Senescence represents the last phase of petal development during which macromolecules and organelles are degraded and nutrients are recycled to developing tissues. To understand better the post-transcriptional changes regulating petal senescence, a proteomic approach was used to profile protein changes during the senescence of Petunia×hybrida ‘Mitchell Diploid’ corollas. Total soluble proteins were extracted from unpollinated petunia corollas at 0, 24, 48, and 72 h after flower opening and at 24, 48, and 72 h after pollination. Two-dimensional gel electrophoresis (2-DE) was used to identify proteins that were differentially expressed in non-senescing (unpollinated) and senescing (pollinated) corollas, and image analysis was used to determine which proteins were up- or down-regulated by the experimentally determined cut-off of 2.1-fold for P <0.05. One hundred and thirty-three differentially expressed protein spots were selected for sequencing. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine the identity of these proteins. Searching translated EST databases and the NCBI non-redundant protein database, it was possible to assign a putative identification to greater than 90% of these proteins. Many of the senescence up-regulated proteins were putatively involved in defence and stress responses or macromolecule catabolism. Some proteins, not previously characterized during flower senescence, were identified, including an orthologue of the tomato abscisic acid stress ripening protein 4 (ASR4). Gene expression patterns did not always correlate with protein expression, confirming that both proteomic and genomic approaches will be required to obtain a detailed understanding of the regulation of petal senescence.
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影响因子：
3.4
作者：
Desclos, Marie;Etienne, Philippe;Avice, Jean-Christophe
通讯作者：
Avice, Jean-Christophe
影响因子：
6.9
作者：
Hoeberichts, Frank A.;van Doorn, Wouter G.;van Wordragen, Monique F.
通讯作者：
van Wordragen, Monique F.
影响因子：
7.4
作者：
Chang, HS;Jones, ML;Clark, DG
通讯作者：
Clark, DG
影响因子：
7.4
作者：
Bhalerao, R;Keskitalo, J;Jansson, S
通讯作者：
Jansson, S
影响因子：
6.5
作者：
Dóczi, R;Kondrák, M;Bánfalvi, Z
通讯作者：
Bánfalvi, Z