Needle-free, spirulina-produced Plasmodium falciparum circumsporozoite vaccination provides sterile protection against pre-erythrocytic malaria in mice.
Needle-free, spirulina-produced Plasmodium falciparum circumsporozoite vaccination provides sterile protection against pre-erythrocytic malaria in mice.
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DOI：
10.1038/s41541-022-00534-5
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发表时间：
2022-10-04
期刊：
影响因子：
9.2
通讯作者：
中科院分区：
文献类型：
--
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抗恶性疟原虫环子孢子蛋白抗体(PfCSP)可阻断子孢子对肝细胞的感染，预防疟疾。无针接种策略是可取的，但大多数以PfCSP为靶向的疫苗，如RTS，S需要针头接种。在这里，我们评估了可食用藻类--钝顶节旋藻(俗称螺旋藻)作为疟疾疫苗平台的作用。通过基因工程使螺旋藻表达由土拨鼠乙肝核心衣壳蛋白(WHcAg)组成的病毒样颗粒(VLP)，并在其表面显示(NANP)15PfCSP抗原。给小鼠鼻腔注射PfCSP-螺旋藻，然后口服PfCSP-螺旋藻增强剂，可产生强烈的系统性抗PfCSP免疫反应，保护小鼠免受表达PfCSP的约氏螺杆菌的皮下攻击。与雄性小鼠不同，雌性小鼠不需要蒙太尼佐剂就能达到高抗体效价或保护作用。螺旋藻作为疫苗输送系统的成功使用，为进一步开发螺旋藻疫苗作为解决疟疾和其他对全球健康具有重要意义的疾病的有用工具提供了保证。
Antibodies against the Plasmodium falciparum circumsporozoite protein (PfCSP) can block hepatocyte infection by sporozoites and protect against malaria. Needle-free vaccination strategies are desirable, yet most PfCSP-targeted vaccines like RTS,S require needle-based administration. Here, we evaluated the edible algae, Arthrospira platensis (commonly called ‘spirulina’) as a malaria vaccine platform. Spirulina were genetically engineered to express virus-like particles (VLPs) consisting of the woodchuck hepatitis B core capsid protein (WHcAg) displaying a (NANP)15 PfCSP antigen on its surface. PfCSP-spirulina administered to mice intranasally followed by oral PfCSP-spirulina boosters resulted in a strong, systemic anti-PfCSP immune response that was protective against subcutaneous challenge with PfCSP-expressing P. yoelii. Unlike male mice, female mice did not require Montanide adjuvant to reach high antibody titers or protection. The successful use of spirulina as a vaccine delivery system warrants further development of spirulina-based vaccines as a useful tool in addressing malaria and other diseases of global health importance.
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