Phosphorous compounds studied by 31P nuclear magnetic resonance spectroscopy in the taenia of guinea‐pig caecum.
Phosphorous compounds studied by 31P nuclear magnetic resonance spectroscopy in the taenia of guinea‐pig caecum.
复制标题
通过 31P 核磁共振波谱研究豚鼠盲肠带内的磷化合物。
DOI：
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
H. Watari
中科院分区：
文献类型：
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作者：
S. Nakayama;Y. Seo;A. Takai;T. Tomita;H. Watari
1.在豚鼠盲肠的分离带（0.4 - 0.6 g）中，在各种代谢条件下使用31 P核磁共振（NMR）研究了细胞内磷化合物和pH值。2.在正常溶液（32 ℃）中，磷酸肌酸（[ PCr]）和无机磷酸盐（[Pi]）与核苷酸三磷酸盐（[ NTP]）的细胞内浓度比值分别为1.71 +/-0.14和0.58 +/-0.11（n = 25）。根据Pi的化学位移估计的细胞内pH值为7.05 +/-0.06（n = 25），与先前获得的结果一致。3.在不存在葡萄糖的情况下，在暴露于40 mM-K+溶液150 min后，[PCr]和[NTP]降低至几乎一半，而[Pi]增加3倍。这些变化比紧张局势的下降速度快得多。当葡萄糖再入院时，对K+的收缩反应在50分钟内完全恢复。然而，这只伴随着[PCr]和[Pi]的部分恢复，但[NTP]没有恢复。细胞内pH值降低约0.2个单位，表明糖酵解增加。4.在无Ca 2+溶液中，缺氧或CN（1 mM）的呼吸抑制仅降低[PCr]，而[NTP]几乎不变。另一方面，在含有Ca 2+（2.4 mM）的过量-K+溶液中的呼吸抑制严重耗尽了PCr，[NTP]降低至40%。将葡萄糖增加到50 mM并不能阻止这些变化，尽管它增加了张力的发展。5. [NTP]和[PCr]在K+肌挛缩时同时降低，提示肌酸磷酸激酶活性低。[PCr]从呼吸抑制中的恢复比[NTP]好得多。通过向标准溶液中加入1 mM腺苷，缓慢但完美地回收了所有NTP峰。6.有人建议，紧张的发展是密切相关的ATP的周转率，而不是其浓度，和腺苷脱氨基是一个限制因素，在ATP的恢复后，过度消费。
1. In the isolated taenia (0.4‐0.6 g) of guinea‐pig caecum, the intracellular phosphorous compounds and pH were investigated using 31P nuclear magnetic resonance (NMR) under various metabolic conditions. 2. The ratios of the intracellular concentration of phosphocreatine ([ PCr]) and inorganic phosphate [( Pi]) to nucleotide triphosphate ([ NTP]) were 1.71 +/‐ 0.14 and 0.58 +/‐ 0.11 (n = 25), respectively, in normal solution (32 degrees C). The intracellular pH estimated from the chemical shift of Pi was 7.05 +/‐ 0.06 (n = 25), agreeing well with those previously obtained. 3. In the absence of glucose, the [PCr] and [NTP] were decreased to almost a half after 150 min exposure to 40 mM‐K+ solution, while [Pi] was increased 3‐fold. These changes were much faster than the rate of decline in tension. When glucose was readmitted, the contractile response to K+ fully recovered in 50 min. However, this was accompanied with only a partial recovery of [PCr] and [Pi], but no recovery of [NTP]. The intracellular pH was lowered by about 0.2 of a unit, suggesting an increase in glycolysis. 4. In Ca2+‐free solution, respiratory inhibition with hypoxia or CN (1 mM) only decreased [PCr], leaving [NTP] nearly unchanged. On the other hand, respiratory inhibition in excess‐K+ solution containing Ca2+ (2.4 mM) severely depleted PCr and decreased [NTP] to 40%. Increasing glucose to 50 mM did not prevent these changes, although it increased tension development. 5. The simultaneous decrease of [NTP] and [PCr] during K+ contracture suggests that the activity of creatine phosphokinase is low. The recovery from respiratory inhibition was much better for [PCr] than for [NTP]. Slow, but perfect, recovery of all NTP peaks was produced by adding 1 mM‐adenosine to normal solution. 6. It was suggested that tension development is closely related to the turnover rate of ATP, and not to its concentration, and that deamination of adenosine is a limiting factor in the recovery of ATP after excessive consumption.
影响因子：
18.2
作者：
T. Butler;M. J. Siegman
通讯作者：
T. Butler;M. J. Siegman
牛蛙胃平滑肌的 1H 和 31P-NMR 研究。
DOI：
10.1016/0167-4889(87)90083-8
发表时间：
1987
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
Yoshizaki,K;Radda,GK;Inubushi,T;Chance,B
通讯作者：
Chance,B
完整细胞和组织中细胞内金属离子的核磁共振研究。
DOI：
10.1146/annurev.bb.13.060184.001253
发表时间：
1984
期刊：
Annual review of biophysics and bioengineering
影响因子：
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作者：
Gupta,RK;Gupta,P;Moore,RD
通讯作者：
Moore,RD