A combined transcriptomics and lipidomics analysis of subcutaneous, epididymal and mesenteric adipose tissue reveals marked functional differences.
A combined transcriptomics and lipidomics analysis of subcutaneous, epididymal and mesenteric adipose tissue reveals marked functional differences.
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DOI：
10.1371/journal.pone.0011525
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发表时间：
2010-07-12
期刊：
影响因子：
3.7
通讯作者：
Drevon CA
中科院分区：
文献类型：
--
作者：
Caesar R;Manieri M;Kelder T;Boekschoten M;Evelo C;Müller M;Kooistra T;Cinti S;Kleemann R;Drevon CA
脂肪组织生理学的贮库依赖性差异可能反映了脂肪细胞和周围组织之间的专门功能和局部相互作用。我们结合时间分辨微阵列分析肠系膜（MWAT），皮下（SWAT）和附睾脂肪组织（EWAT）在高脂肪喂养的雄性转基因ApoE 3莱顿小鼠的组织学，靶向脂质组学和代谢途径的生化分析，以确定差异调节的过程和位点特异性功能。EWAT被发现表现出生理分区。在附睾近端的脂肪中新生脂肪生成稳定地低，而在附睾远端和其他位置的新生脂肪生成在高脂肪饮食中下调。亚油酸和α-亚麻酸的含量在EWAT中比其他仓库有所增加。雄激素受体（Ar）的表达在EWAT高于MWAT和SWAT。我们认为Ar可能介导了从头脂肪生成率的贮库依赖性差异，并提出EWAT中亚油酸和α-亚麻酸的积累受到睾酮介导的从头脂肪生成抑制的影响，并可能促进精子发生过程中这些多不饱和脂肪酸的进一步延长和去饱和。
Depot-dependent differences in adipose tissue physiology may reflect specialized functions and local interactions between adipocytes and surrounding tissues. We combined time-resolved microarray analyses of mesenteric- (MWAT), subcutaneous- (SWAT) and epididymal adipose tissue (EWAT) during high-fat feeding of male transgenic ApoE3Leiden mice with histology, targeted lipidomics and biochemical analyses of metabolic pathways to identify differentially regulated processes and site-specific functions. EWAT was found to exhibit physiological zonation. De novo lipogenesis in fat proximal to epididymis was stably low, whereas de novo lipogenesis distal to epididymis and at other locations was down-regulated in response to high-fat diet. The contents of linoleic acid and α-linolenic acid in EWAT were increased compared to other depots. Expression of the androgen receptor (Ar) was higher in EWAT than in MWAT and SWAT. We suggest that Ar may mediate depot-dependent differences in de novo lipogenesis rate and propose that accumulation of linoleic acid and α-linolenic acid in EWAT is favored by testosterone-mediated inhibition of de novo lipogenesis and may promote further elongation and desaturation of these polyunsaturated fatty acids during spermatogenesis.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
DOI：
10.1073/pnas.0404297101
发表时间：
2004-08-03
影响因子：
11.1
作者：
Chen, GX;Liang, GS;Brown, MS
通讯作者：
Brown, MS
影响因子：
8.7
作者：
Bastelica, D;Morange, P;Alessi, MC
通讯作者：
Alessi, MC
影响因子：
10.8
作者：
Gao, Yu-Jing;Takemori, Kumiko;Lee, Robert M. K. W.
通讯作者：
Lee, Robert M. K. W.
DOI：
10.1073/pnas.231370798
发表时间：
2001-11-20
影响因子：
11.1
作者：
Kawaguchi, T;Takenoshita, M;Uyeda, K
通讯作者：
Uyeda, K