Fluorescent 1,10-phenanthroline-containing oligonucleotides distinguish between perfect and mismatched base pairing.

Fluorescent 1,10-phenanthroline-containing oligonucleotides distinguish between perfect and mismatched base pairing.
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含荧光 1,10-菲咯啉的寡核苷酸可区分完美碱基配对和错配碱基配对。

DOI:
10.1021/ol026043x
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发表时间:
2002
期刊:
影响因子:
5.2
通讯作者:
Tor,Yitzhak
Tor,Yitzhak
中科院分区:
化学1区
文献类型:
--
作者:
Hurley,DennisJ;Seaman,SusanE;Mazura,JanC;Tor,Yitzhak

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A fluorescent deoxyuridine analogue is sensitive to the polarity of its environment and exhibits a distinct emission profile in single- vs double-stranded oligonucleotides. Emission-monitored denaturation curves of internally modified dUphenduplexes are characteristic of the base opposite dUphenand distinguish between perfect and mismatched complementary oligonucleotides.
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