Non-contact measurement of linear external dimensions of the mouse eye.
Non-contact measurement of linear external dimensions of the mouse eye.
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DOI：
10.1016/j.jneumeth.2010.01.006
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发表时间：
2010-03-30
影响因子：
3
通讯作者：
Nickerson, John M.
中科院分区：
文献类型：
--
作者：
Wisard, Jeffrey;Chrenek, Micah A.;Wright, Charles;Dalal, Nupur;Pardue, Machelle T.;Boatright, Jeffrey H.;Nickerson, John M.
对小鼠眼睛数量性状的生物统计分析可以揭示与屈光或眼睛发育相关的异常。由于小鼠眼睛的尺寸较小，因此需要高精度和精确的测量来检测有意义的差异。我们寻求一种非接触式测量技术来获得高度准确和精确的小鼠眼睛的线性尺寸。激光测微法通过量块标准进行了验证。设计了在三个轴上测量眼睛尺寸的简单程序。解剖来自 C57BL/6J 和 C57BL/6J 背景上的 rd10 的小鼠眼睛，并去除眼外肌肉和脂肪。使用激光测微计获得眼轴长度（前后（A-P）轴）和赤道直径（上下（S-I）轴和鼻颞（N-T）轴）的外眼尺寸。采用了多种方法来防止或改善室内空气引起的蒸发。激光测微计的分辨率小于0.77微米，可在三个轴上提供准确、精确的眼睛尺寸非接触式测量。眼睛的外部尺寸与眼睛的重量密切相关。在所收集的几个参数的 28 个配对组合中，眼睛的 N-T 和 S-I 尺寸彼此相关性最为密切。每只小鼠的右眼和左眼的赤道轴测量值相关性良好。每对眼睛的 A-P 测量值不相关或相关性较差。该仪器非常适合测量实验视觉研究和眼科中最常用物种的去核眼睛和其他结构。
Biometric analyses of quantitative traits in eyes of mice can reveal abnormalities related to refractive or ocular development. Due to the small size of the mouse eye, highly accurate and precise measurements are needed to detect meaningful differences. We sought a non-contact measuring technique to obtain highly accurate and precise linear dimensions of the mouse eye. Laser micrometry was validated with gauge block standards. Simple procedures to measure eye dimensions on three axes were devised. Mouse eyes from C57BL/6J and rd10 on a C57BL/6J background were dissected and extraocular muscle and fat removed. External eye dimensions of axial length (anterior-posterior (A-P) axis) and equatorial diameter (superior-inferior (S-I) and nasal-temporal (N-T) axes) were obtained with a laser micrometer. Several approaches to prevent or ameliorate evaporation due to room air were employed. The resolution of the laser micrometer was less than 0.77 microns, and it provided accurate and precise non-contact measurements of eye dimensions on three axes. External dimensions of the eye strongly correlated with eye weight. The N-T and S-I dimensions of the eye correlated with each other most closely from among the 28 pair-wise combinations of the several parameters that were collected. The equatorial axis measurements correlated well from the right and left eye of each mouse. The A-P measurements did not correlate or correlated poorly in each pair of eyes. The instrument is well suited for the measurement of enucleated eyes and other structures from most commonly used species in experimental vision research and ophthalmology.
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影响因子：
2.1
作者：
Dirani, M.;Chamberlain, M.;Baird, P. N.
通讯作者：
Baird, P. N.
影响因子：
64.8
作者：
HILL, RE;FAVOR, J;VANHEYNINGEN, V
通讯作者：
VANHEYNINGEN, V
DOI：
10.1073/pnas.161144098
发表时间：
2001-08-14
影响因子：
11.1
作者：
Collinson, JM;Quinn, JC;Hill, RE
通讯作者：
Hill, RE
影响因子：
1.9
作者：
Jablonski, MM;Wang, XF;Goldowitz, D
通讯作者：
Goldowitz, D
影响因子：
56.9
作者：
GLICKSTEIN, M;MILLODOT, M
通讯作者：
MILLODOT, M