Internalization of the 180 kDa bullous pemphigoid antigen as immune complexes in basal keratinocytes: an important early event in blister formation in bullous pemphigoid
Internalization of the 180 kDa bullous pemphigoid antigen as immune complexes in basal keratinocytes: an important early event in blister formation in bullous pemphigoid
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180 kDa 大疱性类天疱疮抗原作为基底角质形成细胞中的免疫复合物的内化：大疱性类天疱疮形成水疱的重要早期事件
DOI：
10.1046/j.1365-2133.1998.02028.x
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发表时间：
1998
影响因子：
10.3
通讯作者：
Owaribe
中科院分区：
文献类型：
--
作者：
Kitajima;Nojiri;Yamada;Hirako;Owaribe
我们之前已经表明，180 kDa大疱性类天疱疮抗原（BPAG 2）分布在单层培养的角质形成细胞的侧顶（作为池）和腹侧（作为半桥粒）细胞膜上，并且添加从大疱性类天疱疮（BP）患者纯化的IgG（BP-IgG）导致BPAG 2和BP-IgG的免疫复合物从侧顶细胞膜内化。 据信BPAG 2的这种内化抑制腹侧细胞膜上的Ca 2+诱导的半桥粒的再形成，可能是通过抑制抗原从侧顶膜到腹侧膜的供应以形成半桥粒。本文的目的是检查，通过使用BP患者（12例）的活检标本，是否BPAG 2的这种内在化是在原位产生的。 使用针对BPAG 1、BPAG 2和人IgG的单克隆抗体，通过免疫荧光显微镜追踪BPAG 2、230 kDa BPA（BPAG 1）和结合的BP-IgG的命运。在超过一半的病灶和病灶周围活检样本中，观察到BPAG 2内化到基底细胞中，而在正常皮肤中，在基底细胞的整个表面上检测到BPAG 2，但没有内化。在BP和正常表皮中没有检测到BPAG 1的内化。这些结果表明，BP-IgG在基底细胞的侧顶细胞表面上的结合导致BP患者表皮中BPAG 2的原位内化，类似于在培养的细胞系统中观察到的内化，并且BPAG 2和BP-IgG的免疫复合物的这种内化可能在BP的水疱形成中起重要作用。
We have previously shown that the 180 kDa bullous pemphigoid antigen (BPAG2) is distributed on the lateral‐apical (as a pool) and ventral (as hemidesmosomes) cell membranes of monolayer cultured keratinocytes and that addition of IgG purified from bullous pemphigoid (BP) patients (BP‐IgG) causes the internalization of immune complexes of BPAG2 and BP‐IgG from the lateral‐apical cell membrane. This internalization of BPAG2 is believed to inhibit the Ca2+ induced reformation of hemidesmosomes on the ventral cell membrane, possibly by inhibiting the supply of the antigen from the lateral‐apical to the ventral membranes to form hemidesmosomes. The purpose of this paper is to examine, by using biopsy specimens from BP patients (12 cases), whether or not this internalization of BPAG2 is generated in situ. The fates of BPAG2, 230 kDa BPA (BPAG1) and bound BP‐IgG were traced by immunofluorescence microscopy using monoclonal antibodies to BPAG1, BPAG2 and human IgG. In more than half of the lesional and perilesional biopsy specimens, internalization of BPAG2 into the basal cells was observed, while in normal skin BPAG2 was detected on the whole surface of the basal cells without its internalization. No internalization of BPAG1 was detected in BP and normal epidermis. These results suggest that binding of BP‐IgG on the lateral‐apical cell surface of basal cells causes internalization of BPAG2 in situ in the epidermis of BP patients similar to that seen in cultured cell systems, and that this internalization of immune complexes of BPAG2 and BP‐IgG may play an important part in blister formation in BP.
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DOI：
10.1016/s0141-8130(05)80004-2
发表时间：
1992-06-01
影响因子：
8.2
作者：
GREEN, KJ;VIRATA, MLA;PARRY, DAD
通讯作者：
PARRY, DAD
影响因子：
6.5
作者：
GIUDICE, GJ;WILSKE, KC;DIAZ, LA
通讯作者：
DIAZ, LA
人大疱性类天疱疮抗原 (BPAG1)。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sawamura,D;Li,K;Chu,ML;Uitto,J
通讯作者：
Uitto,J
通过亲和纯化的抗体鉴定两组大疱性类天疱疮抗原。
DOI：
10.1111/1523-1747.ep12876194
发表时间：
1990
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Meyer,LJ;Taylor,TB;Kadunce,DP;Zone,JJ
通讯作者：
Zone,JJ
影响因子：
4.4
作者：
Ramzy S. Labib;G. Anhalt;Harikrishna Patel;D. Mutasim;L. Diaz
通讯作者：
Ramzy S. Labib;G. Anhalt;Harikrishna Patel;D. Mutasim;L. Diaz