Myrosin Cell Development Is Regulated by Endocytosis Machinery and PIN1 Polarity in Leaf Primordia of Arabidopsis thaliana[W]
Myrosin Cell Development Is Regulated by Endocytosis Machinery and PIN1 Polarity in Leaf Primordia of Arabidopsis thaliana[W]
复制标题
拟南芥叶原基中Myrosin细胞发育受胞吞机制和PIN1极性调控[W]
DOI：
--
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
I. Hara
中科院分区：
文献类型：
--
作者：
Makoto Shirakawa;H. Ueda;T. Shimada;T. Kohchi;I. Hara
内吞作用依赖的PIN 1在叶原基中的定位决定了特异性地沿沿着的黑芥子素细胞发育，保护植物运输系统。黑芥子细胞，当它们被食草动物破坏时，积累黑芥子酶以产生有毒化合物，在拟南芥中特异性地沿着叶脉形成。然而，这种模式形成的机制尚不清楚。在这里，我们表明，myrosin细胞的发展需要内吞介导的极性定位的生长素外流载体PIN 1在叶原基。内吞/空泡SNARE（syp 22和syp 22 vti 11）的缺陷增强myrosin细胞的发展。通过在PIN 1启动子的控制下表达SYP 22来拯救syp 22表型。此外，myrosin细胞的发展，增强缺乏激活剂的内吞/空泡的RAB 5 GT3（VPS 9A）或PIN 1启动子驱动的表达的显性负性形式的RAB 5 GT3（ARA 7）。相比之下，myrosin细胞的发育不受空泡运输因子的缺陷，包括空泡分选受体VSR 1和逆转录组分VPS 29和VPS 35，这表明内吞途径，而不是空泡运输途径是myrosin细胞发育的重要。不能被极化的拟磷酸化的PIN 1变体（PIN 1-Asp）不仅使myrosin细胞沿沿着形成，而且在脉间叶区域也形成。我们建议，紫茎泽兰目植物可能安排myrosin细胞附近的维管细胞，以保护流动的养分和水分通过极性PIN 1的本地化。
Endocytosis-dependent PIN1 localization in leaf primordia determines myrosin cell development specifically along leaf veins, protecting the plant transport system. Myrosin cells, which accumulate myrosinase to produce toxic compounds when they are ruptured by herbivores, form specifically along leaf veins in Arabidopsis thaliana. However, the mechanism underlying this pattern formation is unknown. Here, we show that myrosin cell development requires the endocytosis-mediated polar localization of the auxin-efflux carrier PIN1 in leaf primordia. Defects in the endocytic/vacuolar SNAREs (syp22 and syp22 vti11) enhanced myrosin cell development. The syp22 phenotype was rescued by expressing SYP22 under the control of the PIN1 promoter. Additionally, myrosin cell development was enhanced either by lacking the activator of endocytic/vacuolar RAB5 GTPase (VPS9A) or by PIN1 promoter-driven expression of a dominant-negative form of RAB5 GTPase (ARA7). By contrast, myrosin cell development was not affected by deficiencies of vacuolar trafficking factors, including the vacuolar sorting receptor VSR1 and the retromer components VPS29 and VPS35, suggesting that endocytic pathway rather than vacuolar trafficking pathway is important for myrosin cell development. The phosphomimic PIN1 variant (PIN1-Asp), which is unable to be polarized, caused myrosin cells to form not only along leaf vein but also in the intervein leaf area. We propose that Brassicales plants might arrange myrosin cells near vascular cells in order to protect the flux of nutrients and water via polar PIN1 localization.
影响因子：
3.3
作者：
Sanderfoot, AA;Kovaleva, V;Raikhel, NV
通讯作者：
Raikhel, NV
DOI：
10.1073/pnas.0711730105
发表时间：
2008-04-22
影响因子：
11.1
作者：
Shroff, Rohit;Vergara, Fredd;Gershenzon, Jonathan
通讯作者：
Gershenzon, Jonathan