Relationship between the Extent of DNA Damage and Gastritis in Normal and Helicobacter pylori-Infected Patients.
Relationship between the Extent of DNA Damage and Gastritis in Normal and Helicobacter pylori-Infected Patients.
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DOI：
10.5009/gnl.2011.5.3.315
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发表时间：
2011-09
期刊：
影响因子：
3.4
通讯作者：
Altahir IE
中科院分区：
文献类型：
--
作者：
Marie MA;Altahir IE
幽门螺杆菌在胃癌发生中的作用尚不清楚，但H。幽门螺杆菌感染被认为是胃癌的易感因素。本研究旨在探讨正常胃粘膜上皮细胞DNA损伤程度与H。幽门阳性和阴性胃炎根据组织学诊断。我们还比较了使用连续孵育时间的胃上皮细胞表面上的彗星状细胞的百分比与胃粘膜细胞下方的百分比。彗星试验是一种快速、有效、重复性好的单细胞DNA损伤检测方法。应用碱性单细胞凝胶电泳（彗星试验）对52例患者的DNA损伤进行了检测。对19例正常人、19例H. pylori阳性和阴性胃炎及14例不同组织学分级的混合病例，以确定组织学诊断与DNA损伤（彗星百分比）之间的关系。正常组织学病例的彗星百分比显著高于H.幽门阳性胃炎。此外，正常和H组胃上皮细胞表面彗星状细胞百分比均显著增加。幽门感染的细胞相比，随后的胃细胞层相同的标本。观察彗星百分率与组织学诊断的关系。
The role of Helicobacter pylori in gastric carcinogenesis is unclear, but H. pylori infection is thought to predispose carriers to gastric cancer. The aim of this study was to investigate the relationship between the extent of DNA damage in normal gastric epithelial cells and H. pylori-positive and -negative gastritis according to histological diagnosis. We also compared the percentage of cometed cells on the surface of the gastric epithelial cells to the percentage beneath the gastric mucosal cells using serial incubations times. The comet assay is a rapid, efficient and reproducible measure of DNA damage in single cell and it was used in this study. DNA damage was evaluated in 52 cases using alkaline single cell gel electrophoresis (comet assay). Comparisons were made between 19 normal individuals, 19 patients with H. pylori-positive and -negative gastritis and 14 mixed cases with different histology gradings to determine if there was a relationship between histological diagnosis and DNA damage (comet percentage). The comet percentages in specimens from cases with normal histology were significantly higher than the comet percentages in specimens from cases with H. pylori-positive gastritis. In addition, there was a significant increase in the percentage of cometed cells on the surface of gastric epithelial cells in both normal and H. pylori-infected cells compared to the subsequent gastric cell layers of the same specimen. A relationship between the comet percentage and the histological diagnosis was observed.
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影响因子：
168.9
作者：
CERUTTI, PA
通讯作者：
CERUTTI, PA
DNA修复基因XRCC3 Thr241Met多态性与中国人群胃癌风险
影响因子：
9.7
作者：
Shen, HB;Wang, XR;Wei, QY
通讯作者：
Wei, QY
影响因子：
3.7
作者：
SINGH, NP;MCCOY, MT;SCHNEIDER, EL
通讯作者：
SCHNEIDER, EL
影响因子：
1.9
作者：
DIXON, MF
通讯作者：
DIXON, MF
DOI：
10.1016/j.mrfmmm.2004.10.006
发表时间：
2005-02-15
影响因子：
2.3
作者：
Arabski, M;Klupinska, G;Blasiak, J
通讯作者：
Blasiak, J