Micromechanical Properties and Energy Requirements of the Microalgae Chlorella vulgaris for Cell Disruption

Micromechanical Properties and Energy Requirements of the Microalgae Chlorella vulgaris for Cell Disruption
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微藻小球藻细胞破碎的微机械特性和能量需求

DOI:
10.1002/ceat.201400632
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发表时间:
2016
影响因子:
2.1
通讯作者:
Kampen
Kampen
中科院分区:
工程技术4区
文献类型:
--
作者:
Günther;Steffi;Gernat;Deborah;Overbeck;Kampen

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细胞破碎是回收细胞内产物的许多过程中不可避免的步骤。目前,这些工艺仍然是能源密集型和成本效益低的,特别是对于低成本的散装产品,如来自微藻的生物柴油。采用纳米压痕仪对普通小球藻单细胞进行压缩试验,研究了细胞破碎特性。结果表明,随着渗透压的增加,爆裂力和能量增加,爆裂细胞的百分比下降。显微镜水平的观察结果也可以使用搅拌介质研磨机进行细胞破碎转移到宏观水平。将细胞破碎的结果与压缩试验的结果进行比较,并在能量方面进行评价。
Cell disruption is an inevitable step in many processes for the recovery of intracellular products. Currently, those processes are still energy‐intensive and cost‐ineffective, in particular with regard to low‐cost bulk products such as biodiesel from microalgae. Characteristics of cell disruption were studied by compressive testing of single cells ofChlorella vulgariswith a nanoindenter. Results showed that bursting force as well as energy increased and the percentage of burst cells decreased with higher osmolality. The observations on microscopic level could also be transferred on macroscopic level using a stirred‐media mill for cell disruption. The results of the cell disruption were compared to the findings of the compression tests and evaluated under energetic aspects.
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