Chronic nitric oxide synthase inhibition exacerbates renal dysfunction in cirrhotic rats.
Chronic nitric oxide synthase inhibition exacerbates renal dysfunction in cirrhotic rats.
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慢性一氧化氮合酶抑制会加剧肝硬化大鼠的肾功能障碍。
DOI：
10.1152/ajprenal.00089.2003
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
T. Jonassen
中科院分区：
文献类型：
--
作者：
M. Graebe;L. Brønd;S. Christensen;S. Nielsen;N. Olsen;T. Jonassen
本文研究了慢性一氧化氮（NO）系统阻断的肝硬化大鼠的钠平衡和肾小管功能。大鼠于胆总管结扎（CBL）当日开始给予非选择性NO合成酶抑制剂ng -硝基-l-精氨酸甲酯（l-NAME）治疗。三周的每日钠平衡研究表明，与假手术大鼠相比，CBL大鼠出现钠潴留，l-NAME治疗剂量依赖性地通过减少尿钠排泄来恶化累积钠平衡。CBL后5周，进行肾脏清除率研究，随后对电中性3型钠/质子交换剂（NHE3）和近端小管中的na - k - atp酶进行Western blot检测。未经治疗的CBL大鼠显示近端重吸收减少，同时NHE3和na - k - atp酶水平降低，表明近端小管远端管段是钠重吸收增加的原因。l- name处理的CBL大鼠通过锂清除法测量近端重吸收增加，NHE3和na - k - atp酶蛋白水平显着增加。我们的研究结果表明，慢性l-NAME治疗通过显著增加近端小管重吸收而加剧了CBL大鼠的钠潴留。
The present study investigated sodium balance and renal tubular function in cirrhotic rats with chronic blockade of the nitric oxide (NO) system. Rats were treated with the nonselective NO synthase inhibitor NG-nitro-l-arginine methyl ester (l-NAME) starting on the day of common bile duct ligation (CBL). Three weeks of daily sodium balance studies showed that CBL rats developed sodium retention compared with sham-operated rats and that l-NAME treatment dose dependently deteriorated cumulative sodium balance by reducing urinary sodium excretion. Five weeks after CBL, renal clearance studies were performed, followed by Western blotting of the electroneutral type 3 sodium/proton exchanger (NHE3) and the Na-K-ATPase present in proximal tubules. Untreated CBL rats showed a decreased proximal reabsorption with a concomitant reduction of NHE3 and Na-K-ATPase levels, indicating that tubular segments distal to the proximal tubules were responsible for the increased sodium reabsorption. l-NAME-treated CBL rats showed an increased proximal reabsorption measured by the lithium clearance method and showed a marked increase in NHE3 and Na-K-ATPase protein levels. Our results show that chronic l-NAME treatment exacerbates the sodium retention found in CBL rats by a significant increase in proximal tubular reabsorption.
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DOI：
10.1152/ajpregu.1996.271.1.r295
发表时间：
1996-07
期刊：
The American journal of physiology
影响因子：
--
作者：
G. Dibona;L. Sawin;S. Jones
通讯作者：
G. Dibona;L. Sawin;S. Jones
DOI：
10.1152/ajprenal.1998.274.5.f946
发表时间：
1998-05
期刊：
American journal of physiology. Renal physiology
影响因子：
--
作者：
Ming Lu;Xiaohong Wang;Wen‐Hui Wang
通讯作者：
Ming Lu;Xiaohong Wang;Wen‐Hui Wang
DOI：
10.1172/jci119531
发表时间：
1997-07
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
H. Siragy;Robert M. Carey
通讯作者：
H. Siragy;Robert M. Carey
肝硬化大鼠的神经元一氧化氮合酶和全身血管舒张。
DOI：
10.1152/ajprenal.2000.279.6.f1110
发表时间：
2000
期刊：
American journal of physiology. Renal physiology
影响因子：
--
作者：
Xu,L;Carter,EP;Ohara,M;Martin,PY;Rogachev,B;Morris,K;Cadnapaphornchai,M;Knotek,M;Schrier,RW
通讯作者：
Schrier,RW
DOI：
10.1073/pnas.95.24.14552
发表时间：
1998-11-24
影响因子：
11.1
作者：
Kim, GH;Masilamani, S;Knepper, MA
通讯作者：
Knepper, MA