Targeted nanoparticles enhanced flow electroporation of antisense oligonucleotides in leukemia cells.
Targeted nanoparticles enhanced flow electroporation of antisense oligonucleotides in leukemia cells.
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DOI：
10.1016/j.bios.2010.06.025
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发表时间：
2010-10-15
影响因子：
12.6
通讯作者：
Lee LJ
中科院分区：
文献类型：
--
作者：
Wang S;Zhang X;Yu B;Lee RJ;Lee LJ
Liposome nanoparticles (LNs) with a targeting ligand were used in a semi-continuous flow electroporation (SFE) device to enhance in vitro delivery of exogenous oligonucleotides (ODN). Nanoparticles comprising transferrin-targeted lipoplex encapsulating ODN G3139 were mixed with K562 cells (a chronic myeloid leukemia cell line) and incubated for half an hour to accomplish nanoparticle binding. The mixture was then flowed through a SFE channel where electric pulses were given. Better ODN delivery efficiency was achieved with an increase of ~24% to the case in combination of non-targeted LNs and SFE, and ~60% to the case using targeted LNs alone, respectively. The MTS assay results confirmed cell viability greater than 75%.
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