CARK1 phosphorylates subfamily III members of ABA receptors.
CARK1 phosphorylates subfamily III members of ABA receptors.
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CARK1 磷酸化 ABA 受体亚家族 III 成员
DOI：
10.1093/jxb/ery374
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发表时间：
2019-01-07
影响因子：
6.9
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Li X;Kong X;Huang Q;Zhang Q;Ge H;Zhang L;Li G;Peng L;Liu Z;Wang J;Li X;Yang Y
CARK1优先与III亚家族的ABA受体相互作用并磷酸化；磷酸化位点RCAR11T78在ABA反应通路的激活中起着重要作用。脱落酸（ABA）在植物对非生物胁迫的反应中起着至关重要的作用，使植物能够应对环境的挑战。在这项研究中，我们分析了III亚家族的ABA受体作为胞质ABA受体激酶1 （CARK1）的潜在靶点。我们之前发现，CARK1在一个独特的苏氨酸残基上磷酸化了亚家族III成员RCAR11 （T78）。我们的研究现在显示了CARK1与受体RCAR12/13/14在体外和体内的物理相互作用。催化活性不强的CARK1-N204A不与受体相互作用。这些ABA受体的体外磷酸化发生在RCAR11中对应于T78的丝氨酸/苏氨酸氨基酸残基上，该残基位于一个保守位点的β3环内。进一步分析发现，RCAR11T78的磷酸化可以提高pyr1pyl1pyl2pyl4四重突变体（1124）对ABA的敏感性，包括抑制根伸长和提高耐旱性。CARK1:1124的互补和ABA相关基因的表达分析表明，CARK1可以挽救1124对ABA的不敏感。我们的研究结果表明，CARK1有磷酸化亚家族III ABA受体的倾向，而磷酸化位点RCAR11T78、RCAR12T105、RCAR13T101和RCAR14S81是参与ABA反应通路激活的主要位点。
CARK1 preferentially interacts with and phosphorylates ABA receptors of subfamily III; the phosphorylation site RCAR11T78 plays a substantial role in the activation of the ABA response pathway. Abscisic acid (ABA) plays a vital role in responses to abiotic stresses that allow plants to cope with environmental challenges. In this study, we analyzed ABA receptors of subfamily III as the potential targets of Cytosolic ABA Receptor Kinase 1 (CARK1). We previously found that CARK1 phosphorylated the subfamily III member RCAR11 at a distinct threonine residue (T78). Our study now shows the physical interaction of CARK1 with the receptors RCAR12/13/14 in vitro and in vivo. The catalytically inactive form CARK1-N204A did not interact with the receptors. Phosphorylation of these ABA receptors in vitro occurred at a serine/threonine amino acid residue corresponding to T78 in RCAR11, which is located in the loop of β3 within a conserved site. Further analysis revealed that the phosphorylation of RCAR11T78 could increase the sensitivity of the pyr1pyl1pyl2pyl4 quadruple mutant (1124) to ABA, including the inhibition of root elongation and increasing drought tolerance. The analysis of CARK1:1124 complementation and the expression of ABA-related genes indicated that CARK1 could rescue the insensitivity of 1124 to ABA. Our results indicate that CARK1 tends to phosphorylate subfamily III ABA receptors, and the phosphosites RCAR11T78, RCAR12T105, RCAR13T101, and RCAR14S81 are the major sites involved in the activation of the ABA response pathway.
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DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
影响因子：
16.8
作者：
Yin, Ping;Fan, He;Yan, Nieng
通讯作者：
Yan, Nieng
DOI：
10.1073/pnas.0505667103
发表时间：
2006-02-07
影响因子：
11.1
作者：
Furihata, T;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
64.8
作者：
Miyazono, Ken-ichi;Miyakawa, Takuya;Tanokura, Masaru
通讯作者：
Tanokura, Masaru