Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins.
Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins.
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DOI：
10.1126/science.1173041
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发表时间：
2009-05-22
期刊：
影响因子：
--
通讯作者：
Cutler SR
中科院分区：
文献类型：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
对合成阿坝激动剂的分析揭示了一个新的阿坝结合蛋白家族，该家族通过直接调节2C型蛋白磷酸酶的活性来控制信号转导。PP 2Cs是重要的磷酸酶，在脱落酸（阿坝）信号转导中起重要作用。使用化学遗传学，我们以前确定了一种称为pyrabactin的合成生长抑制剂。在这里，我们表明，pyrabactin是一种选择性的阿坝激动剂，通过PYR 1，一个家庭的START蛋白质称为PYR/PYLs，这是必要的pyrabactin和阿坝信号在体内的创始成员。我们发现阿坝与PYR 1结合，PYR 1反过来又结合并抑制PP 2C。因此，我们认为PYR/PYLs是ABA受体，其在通过抑制PP 2Cs来控制阿坝信号传导的负调节途径的顶点起作用。我们的结果说明了小分子方法在避免阻碍遗传分析的功能冗余方面的作用。
Analysis of a synthetic ABA agonist uncovers a new family of ABA binding proteins that control signal transduction by directly regulating the activity of type 2C protein phosphatases. PP2Cs are vital phosphatases that play important roles in abscisic acid (ABA) signaling. Using chemical genetics, we previously identified a synthetic growth inhibitor called pyrabactin. Here we show that pyrabactin is a selective ABA agonist that acts through PYR1, the founding member of a family of START proteins called PYR/PYLs, which are necessary for both pyrabactin and ABA signaling in vivo. We show that ABA binds to PYR1, which in turn binds to and inhibits PP2Cs. We therefore suggest that PYR/PYLs are ABA-receptors that function at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting PP2Cs. Our results illustrate the power of small-molecule approaches for sidestepping the functional redundancy that hampers genetic analysis.
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