Optical molecular imaging of lysyl oxidase activity - detection of active fibrogenesis in human lung tissue.
Optical molecular imaging of lysyl oxidase activity - detection of active fibrogenesis in human lung tissue.
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DOI：
10.1039/c5sc01258a
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发表时间：
2015-08-01
期刊：
影响因子：
8.4
通讯作者：
Dhaliwal K
中科院分区：
文献类型：
--
作者：
Aslam T;Miele A;Chankeshwara SV;Megia-Fernandez A;Michels C;Akram AR;McDonald N;Hirani N;Haslett C;Bradley M;Dhaliwal K
荧光探针提供实时测量赖氨酸氧化酶活性在离体动物和人肺组织。异常纤维形成是许多多器官系统疾病的特征。赖氨酸氧化酶家族对组织稳态至关重要，赖氨酸氧化酶活性的升高与纤维增殖和癌间质有关。我们已经合成了一种新的荧光报告基因，用于监测赖氨酸氧化酶的活性，在体外通气纤维化的猪肺和体外人肺组织中，探针激活后荧光增加3-5倍。被称为“oLOX”的探针可以在许多生物环境中提供赖氨酸氧化酶活性的实时测量，并且从体外环境到人体都易于处理。
A fluorogenic probe provides real-time measurement of lysyl oxidase activity in ex vivo asinine and human lung tissue. Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man.
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影响因子：
15
作者：
Dickinson BC;Huynh C;Chang CJ
通讯作者：
Chang CJ
影响因子：
4.9
作者：
Albers, Aaron E.;Rawls, Katherine A.;Chang, Christopher J.
通讯作者：
Chang, Christopher J.
DOI：
10.1186/1755-1536-2-7
发表时间：
2009-12-15
期刊：
Fibrogenesis & tissue repair
影响因子：
--
作者：
Chen CZ;Raghunath M
通讯作者：
Raghunath M
影响因子：
4.1
作者：
Aw, Junxin;Shao, Qing;Xing, Bengang
通讯作者：
Xing, Bengang
影响因子：
4.8
作者：
Borel, A;Eichenberger, D;Font, B
通讯作者：
Font, B