Breeding without breeding: is a complete pedigree necessary for efficient breeding?
Breeding without breeding: is a complete pedigree necessary for efficient breeding?
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DOI：
10.1371/journal.pone.0025737
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Lstibůrek M
中科院分区：
文献类型：
--
作者：
El-Kassaby YA;Cappa EP;Liewlaksaneeyanawin C;Klápště J;Lstibůrek M
完整的系谱信息是现代育种和对父母和后代进行选择和部署决策的先决条件。DNA指纹和系谱重建可以通过对自然产生的后代的亲缘关系描述来代替人工交配。在这里，我们报告了一种称为“无繁殖繁殖”（BwB）的育种概念的有效性，该概念绕过人工交配，而是专注于随机抽样的子集，母体已知但父亲未知的后代来描绘其父系关系。然后，我们使用构建谱系的完整（全同胞：FS）和不完整（半同胞：HS）分析的组合，生成对这些后代及其父系父母进行排名所需的信息。研究人员对在41个亲本西部落叶松种群中生长的15个雌性（种子供体）风媒传粉后代的随机样本进行了评估，并将其与两种常用的测试方法进行了比较，这两种测试方法依赖于不完全（母亲同父异母，开放传粉：OP）或完全（FS）谱系设计。BwB产生的结果优于不完整设计的结果，与完整谱系方法产生的结果几乎相同。结合使用完整和不完整的谱系信息，可以评估所有父母，包括母亲和父亲，以及所有的后代，这是单独使用OP或FS方法无法完成的结果。我们还从指纹后代的比例、双亲同父异母家庭的规模、外部基因流的作用和自交，以及可以用BwB实际测试的父母数量等方面讨论了最佳实验环境。
Complete pedigree information is a prerequisite for modern breeding and the ranking of parents and offspring for selection and deployment decisions. DNA fingerprinting and pedigree reconstruction can substitute for artificial matings, by allowing parentage delineation of naturally produced offspring. Here, we report on the efficacy of a breeding concept called “Breeding without Breeding” (BwB) that circumvents artificial matings, focusing instead on a subset of randomly sampled, maternally known but paternally unknown offspring to delineate their paternal parentage. We then generate the information needed to rank those offspring and their paternal parents, using a combination of complete (full-sib: FS) and incomplete (half-sib: HS) analyses of the constructed pedigrees. Using a random sample of wind-pollinated offspring from 15 females (seed donors), growing in a 41-parent western larch population, BwB is evaluated and compared to two commonly used testing methods that rely on either incomplete (maternal half-sib, open-pollinated: OP) or complete (FS) pedigree designs. BwB produced results superior to those from the incomplete design and virtually identical to those from the complete pedigree methods. The combined use of complete and incomplete pedigree information permitted evaluating all parents, both maternal and paternal, as well as all offspring, a result that could not have been accomplished with either the OP or FS methods alone. We also discuss the optimum experimental setting, in terms of the proportion of fingerprinted offspring, the size of the assembled maternal and paternal half-sib families, the role of external gene flow, and selfing, as well as the number of parents that could be realistically tested with BwB.
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影响因子：
2.2
作者：
Funda, Tomas;Liewlaksaneeyanawin, Cherdsak;El-Kassaby, Yousry A.
通讯作者：
El-Kassaby, Yousry A.
影响因子：
2.4
作者：
Hansen, Ole K.;McKinney, Lea V.
通讯作者：
McKinney, Lea V.
影响因子：
5.4
作者：
Lambeth, C;Lee, BC;Wheeler, N
通讯作者：
Wheeler, N
DOI：
10.1111/j.1471-8286.2006.01291.x
发表时间：
2006-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Isoda, Keiya;Watanabe, Atsushi
通讯作者：
Watanabe, Atsushi
影响因子：
5.4
作者：
Grattapaglia, D;Ribeiro, VJ;Rezende, GDSP
通讯作者：
Rezende, GDSP