Comparative genomic analysis and virulence differences in closely related salmonella enterica serotype heidelberg isolates from humans, retail meats, and animals.
Comparative genomic analysis and virulence differences in closely related salmonella enterica serotype heidelberg isolates from humans, retail meats, and animals.
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DOI：
10.1093/gbe/evu079
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发表时间：
2014-05
影响因子：
3.3
通讯作者：
McDermott PF
中科院分区：
文献类型：
--
作者：
Hoffmann M;Zhao S;Pettengill J;Luo Y;Monday SR;Abbott J;Ayers SL;Cinar HN;Muruvanda T;Li C;Allard MW;Whichard J;Meng J;Brown EW;McDermott PF
肠道沙门氏菌肠道亚种肠道血清型海德堡（S. Heidelberg）是引起人类沙门氏菌病的顶级血清型之一。最近，这种血清型的一种耐药性菌株与2011年因食用受污染的火鸡而引起的多州大爆发有关，涉及136例确诊病例，其中1人死亡。在这项研究中，我们评估了44 S的进化多样性。使用454 GS FLX（Roche）平台生成的全基因组测序（WGS）的海德堡分离株。这些分离株，包括30个几乎无法区分（一个带差）的Xbal脉冲场凝胶电泳图谱（JF6X01.0032，JF6X01.0058），是在1982年至2011年期间从不同来源收集的，其中包括9个与2011年爆发相关的分离株。此外，我们使用Pacific Biosciences（PacBio）系统确定了与2011年爆发相关的临床分离株的染色体和三种质粒的完整序列。使用单核苷酸多态性（SNP）分析，我们能够区分高度克隆的菌株，包括在同一年的不同时间分离的菌株。来自最近2011年爆发的分离株聚集在一起，平均SNP变异仅为17个SNP。色葡萄海德堡分离物携带多种病原菌，如前噬菌体P22、P4、类芽孢杆菌前噬菌体Giffy-2和携带sopE 1基因的类P2噬菌体，包括62个致病性基因，以及13个菌毛标记和不亲和（Inc）I1、印加/C和IncHI 2群的抗性质粒。21株含有携带IV型分泌系统的IncX质粒。在本研究中使用的最近和历史分离株的基础上，我们的结果表明，除了提供分离株的详细遗传信息外，WGS还可以鉴定可用于区分高度克隆S的SNP靶。海德堡分离物。
Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg) is one of the top serovars causing human salmonellosis. Recently, an antibiotic-resistant strain of this serovar was implicated in a large 2011 multistate outbreak resulting from consumption of contaminated ground turkey that involved 136 confirmed cases, with one death. In this study, we assessed the evolutionary diversity of 44 S. Heidelberg isolates using whole-genome sequencing (WGS) generated by the 454 GS FLX (Roche) platform. The isolates, including 30 with nearly indistinguishable (one band difference) Xbal pulsed-field gel electrophoresis patterns (JF6X01.0032, JF6X01.0058), were collected from various sources between 1982 and 2011 and included nine isolates associated with the 2011 outbreak. Additionally, we determined the complete sequence for the chromosome and three plasmids from a clinical isolate associated with the 2011 outbreak using the Pacific Biosciences (PacBio) system. Using single-nucleotide polymorphism (SNP) analyses, we were able to distinguish highly clonal isolates, including strains isolated at different times in the same year. The isolates from the recent 2011 outbreak clustered together with a mean SNP variation of only 17 SNPs. The S. Heidelberg isolates carried a variety of phages, such as prophage P22, P4, lambda-like prophage Gifsy-2, and the P2-like phage which carries the sopE1 gene, virulence genes including 62 pathogenicity, and 13 fimbrial markers and resistance plasmids of the incompatibility (Inc)I1, IncA/C, and IncHI2 groups. Twenty-one strains contained an IncX plasmid carrying a type IV secretion system. On the basis of the recent and historical isolates used in this study, our results demonstrated that, in addition to providing detailed genetic information for the isolates, WGS can identify SNP targets that can be utilized for differentiating highly clonal S. Heidelberg isolates.
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影响因子：
2
作者：
Chittick, P;Sulka, A;Fry, AM
通讯作者：
Fry, AM
DOI：
10.1099/00221287-137-3-601
发表时间：
1991-03-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
BELTRAN, P;PLOCK, SA;SELANDER, RK
通讯作者：
SELANDER, RK
DOI：
10.1016/j.future.2006.07.009
发表时间：
2007-03-01
影响因子：
7.5
作者：
Bazinet, Adam L.;Myers, Daniel S.;Cummings, Michael P.
通讯作者：
Cummings, Michael P.
影响因子：
9.2
作者：
Aballay, A;Yorgey, P;Ausubel, FM
通讯作者：
Ausubel, FM
影响因子：
3.7
作者：
Darling AE;Mau B;Perna NT
通讯作者：
Perna NT