Highly parallel SNP genotyping reveals high-resolution landscape of mono-allelic Ube3a expression associated with locus-wide antisense transcription.
Highly parallel SNP genotyping reveals high-resolution landscape of mono-allelic Ube3a expression associated with locus-wide antisense transcription.
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DOI：
10.1093/nar/gkq1201
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发表时间：
2011-04
影响因子：
14.9
通讯作者：
Kiyosawa H
中科院分区：
文献类型：
--
作者：
Numata K;Kohama C;Abe K;Kiyosawa H
我们通过高度平行的SNP基因分型平台研究了小鼠Ube3a（泛素蛋白连接酶E3A）的兆碱基宽基因组区域的等位基因和链特异性转录景观。我们已经成功地确定了母亲特异性表达的Ube3a和它的反义对应物（Ube3a-ATS）在大脑中，但不是在肝脏。由于使用了亚种间杂交小鼠，这种大碱基范围的分析提供了该区域转录模式的高分辨率图像。首先，我们发现，脑特异性的母亲表达的Ube3a是限制在第二半部分的轨迹，但不存在从第一半部分。等位基因表达的平衡在基因座的中间被改变。其次，我们发现脑特异性Ube3a-ATS的表达似乎终止于Ube3a转录起始位点上游区域。本研究强调了有义和反义转录本之间全基因座竞争的重要性。
We investigated the allele- and strand-specific transcriptional landscape of a megabase-wide genomic region of mouse Ube3a (ubiquitin protein ligase E3A) by means of a highly parallel SNP genotyping platform. We have successfully identified maternal-specific expression of Ube3a and its antisense counterpart (Ube3a-ATS) in brain, but not in liver. Because of the use of inter-subspecies hybrid mice, this megabase-wide analysis provided high-resolution picture of the transcriptional patterns of this region. First, we showed that brain-specific maternal expression of Ube3a is restricted to the second half part of the locus, but is absent from the first half part. Balance of allelic expression is altered in the middle of the locus. Second, we showed that expression of the brain-specific Ube3a-ATS appeared to be terminated in the region upstream to the Ube3a transcription start site. The present study highlights the importance of locus-wide competition between sense and antisense transcripts.
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影响因子：
4.4
作者：
Numata K;Osada Y;Okada Y;Saito R;Hiraiwa N;Nakaoka H;Yamamoto N;Watanabe K;Okubo K;Kohama C;Kanai A;Abe K;Kiyosawa H
通讯作者：
Kiyosawa H
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10.5
作者：
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通讯作者：
Tilghman, Shirley M.
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Kishino, T.
通讯作者：
Kishino, T.
影响因子：
14.9
作者：
Landers, M;Bancescu, DL;Lalande, M
通讯作者：
Lalande, M