Differential localization of vesicular glutamate transporters and peptides in corneal afferents to trigeminal nucleus caudalis.
Differential localization of vesicular glutamate transporters and peptides in corneal afferents to trigeminal nucleus caudalis.
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DOI：
10.1002/cne.22414
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发表时间：
2010-09-01
期刊：
影响因子：
--
通讯作者：
Aicher SA
中科院分区：
文献类型：
--
作者：
Hegarty DM;Tonsfeldt K;Hermes SM;Helfand H;Aicher SA
三叉神经事件将伤害性信息从角膜表面传递到三叉神经尾侧亚核（Vc）。像其他伤害感受器一样，三叉神经传入被认为使用谷氨酸和神经肽作为神经递质。目前的研究检查了角膜传入是否同时包含神经肽和囊状谷氨酸转运蛋白。用霍乱毒素B （CTb）鉴定角膜对Vc的传入。角膜事件以两簇方式投射到Vc的吻侧和尾侧边界；包含功能不同的伤害神经元的区域。因此，分别检查投射到这两个区域的角膜事件。双重免疫细胞化学研究将CTb与降钙素基因相关肽（CGRP）、P物质（SP）、泡状谷氨酸转运蛋白1 （VGluT1）或VGluT2联合使用。与SP相比，角膜事件更可能含有CGRP，而向吻侧区域投射的角膜事件比向尾侧区域投射的角膜事件更可能含有CGRP。总的来说，角膜事件同样可能含有VGluT1或VGluT2。总的来说，61%的角膜传入事件含有VGluT1或VGluT2，这表明一些传入事件缺乏水疱性谷氨酸转运蛋白。尾侧角膜事件中含有VGluT2的可能性大于VGluT1；而吻侧角膜事件中含有VGluT1的可能性大于VGluT2。联合CTb、CGRP和VGluT2的三重标记研究显示，很少有角膜事件同时含有CGRP和VGluT2；尾端（1%）和喙端（2%）。这些结果表明，大多数角膜事件含有肽或VGluT，但很少两者都含有。我们的结果与越来越多的文献一致，表明谷氨酸能和肽能感觉传入可能是不同的群体。
Trigeminal afferents convey nociceptive information from the corneal surface of the eye to trigeminal subnucleus caudalis (Vc). Trigeminal afferents, like other nociceptors, are thought to use glutamate and neuropeptides as neurotransmitters. The current studies examined whether corneal afferents contain both neuropeptides and vesicular glutamate transporters. Corneal afferents to Vc were identified using Cholera Toxin B (CTb). Corneal afferents project in two clusters to the rostral and caudal borders of Vc; regions that contain functionally distinct nociceptive neurons. Thus, corneal afferents projecting to these two regions were examined separately. Dual immunocytochemical studies combined CTb with either calcitonin gene-related peptide (CGRP), substance P (SP), vesicular glutamate transporter 1 (VGluT1) or VGluT2. Corneal afferents were more likely to contain CGRP than SP, and corneal afferents projecting to the rostral region were more likely to contain CGRP than afferents projecting caudally. Overall, corneal afferents were equally likely to contain VGluT1 or VGluT2. Together, 61% of corneal afferents contained either VGluT1 or VGluT2, suggesting that some afferents lack a vesicular glutamate transporter. Caudal corneal afferents were more likely to contain VGluT2 than VGluT1; while rostral corneal afferents were more likely to contain VGluT1 than VGluT2. Triple labeling studies combining CTb, CGRP and VGluT2 showed very few corneal afferents contain both CGRP and VGluT2; caudally (1%) and rostrally (2%). These results suggest that most corneal afferents contain a peptide or a VGluT, but rarely both. Our results are consistent with a growing literature suggesting that glutamatergic and peptidergic sensory afferents may be distinct populations.
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DOI：
10.1073/pnas.76.7.3532
发表时间：
1979-01-01
影响因子：
11.1
作者：
CUELLO, AC;GALFRE, G;MILSTEIN, C
通讯作者：
MILSTEIN, C
影响因子：
2.9
作者：
Aicher, SA;Mitchell, JL;Zadina, JE
通讯作者：
Zadina, JE
影响因子：
3.3
作者：
Meng, ID;Bereiter, DA
通讯作者：
Bereiter, DA
影响因子：
2.5
作者：
LLEWELLYNSMITH, IJ;PILOWSKY, P;CHALMERS, J
通讯作者：
CHALMERS, J
影响因子：
2.5
作者：
Melone, M;Burette, A;Weinberg, RJ
通讯作者：
Weinberg, RJ