Characterization and purification of a secreted plasminogen activator inhibitor (PAI-1) induced by transforming growth factor-beta 1 in normal rat kidney (NRK) cells: decreased PAI-1 expression in transformed NRK cells.
Characterization and purification of a secreted plasminogen activator inhibitor (PAI-1) induced by transforming growth factor-beta 1 in normal rat kidney (NRK) cells: decreased PAI-1 expression in transformed NRK cells.
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正常大鼠肾 (NRK) 细胞中转化生长因子-β1 诱导的分泌型纤溶酶原激活剂抑制剂 (PAI-1) 的表征和纯化：转化的 NRK 细胞中 PAI-1 表达降低。
DOI：
10.1210/endo-126-6-2936
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发表时间：
1990
期刊：
影响因子：
4.8
通讯作者：
J. Keski‐oja
中科院分区：
文献类型：
--
作者：
M. J. Newman;E. Lane;A. Iannotti;Matthew A. Iannotti;R. Pepinsky;J. Keski‐oja
1型转化生长因子- β (TGF β 1)被发现是正常大鼠肾（NRK）细胞分泌49,000 mol wt蛋白的有效诱导剂。根据分子质量、十二烷基硫酸钠存在下的活性、PAI-1抗体的免疫沉淀和n端序列分析，该蛋白与1型纤溶酶原激活物抑制剂（PAI-1）相关。当细胞与皮摩尔浓度的TGF β 1孵育24 h时，NRK细胞条件培养基中的PAI-1水平增加了5- 11倍，浓度约为0.3微克/毫升。分泌的PAI-1沉积在NRK细胞外基质中，并释放到培养基中。在不存在TGF β 1或TGF β 1存在的情况下，自发转化的NRK细胞系分泌的PAI-1比亲本细胞系少3-4倍，而在Kirsten肉瘤病毒转化的NRK细胞中，PAI-1的分泌几乎完全消失。建立了一种新的纯化工艺，分离出高活性、不含洗涤剂的TGF β 1诱导的PAI-1。
Type 1 transforming growth factor-beta (TGF beta 1) was found to be a potent inducer of the secretion of a 49,000 mol wt protein by normal rat kidney (NRK) cells. This protein was related to type 1 plasminogen activator inhibitor (PAI-1) on the basis of molecular mass, activity in the presence of sodium dodecyl sulfate, immunoprecipitation by antibodies to PAI-1, and N-terminal sequence analysis. PAI-1 levels in the conditioned medium of NRK cells were increased 5- to 11-fold when cells were incubated with picomolar concentrations of TGF beta 1 for 24 h, reaching a concentration of approximately 0.3 microgram/ml. The secreted PAI-1 was deposited in the NRK extracellular matrix as well as released into the culture medium. A spontaneously transformed NRK cell line was found to secrete 3-4 times less PAI-1, in the absence or presence of TGF beta 1, compared to the parent cell line, while PAI-1 secretion in Kirsten sarcoma virus-transformed NRK cells was almost completely abrogated. A novel purification procedure was established, which results in the isolation of highly active and detergent-free TGF beta 1-induced PAI-1.
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DOI：
10.1016/s0021-9258(17)42691-3
发表时间：
1984-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. V. van Mourik;D. Lawrence;D. Loskutoff
通讯作者：
J. V. van Mourik;D. Lawrence;D. Loskutoff
DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
反向纤维蛋白自显影：十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后检测和部分表征蛋白酶抑制剂的方法。
影响因子：
2.9
作者：
Erickson,LA;Lawrence,DA;Loskutoff,DJ
通讯作者：
Loskutoff,DJ
DOI：
--
发表时间：
1986-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ignotz;J. Massagué
通讯作者：
R. Ignotz;J. Massagué
内皮细胞产生纤溶酶原激活剂的潜在抑制剂，可以被变性剂激活。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hekman,CM;Loskutoff,DJ
通讯作者：
Loskutoff,DJ