Venom from the scorpion Heterometrus liangi inhibits HeLa cell proliferation by inducing p21 expression
Venom from the scorpion Heterometrus liangi inhibits HeLa cell proliferation by inducing p21 expression
复制标题
蝎子 Heterometrus liangi 的毒液通过诱导 p21 表达抑制 HeLa 细胞增殖
DOI：
10.2478/s11756-018-0119-1
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发表时间：
2018-09
期刊：
影响因子：
1.5
通讯作者：
Qisheng Song
中科院分区：
文献类型：
--
作者：
Juan Wang;Yu;e Peng;Zhi Wang;Xiangping Chai;Zhiyue Lv;Qisheng Song
蝎毒是鉴定和开发用于癌症治疗的有前途的生物分子的重要来源。为寻找有应用前景的肿瘤生物分子，研究了梁氏异蝎粗毒（HLSN）对HeLa细胞增殖、凋亡的影响，并与东亚钳蝎（BMK）进行了比较。采用RT-PCR、Western blot和免疫组化方法检测p21蛋白在对照组和处理组细胞中的表达水平。结果表明，HLSN蛇毒作用HeLa细胞24和48 h后，MTT法显示蛇毒呈剂量依赖性地抑制HeLa细胞增殖，HE染色、扫描电镜和透射电镜观察显示蛇毒对HeLa细胞有明显的形态学改变，流式细胞术（FCM）检测细胞凋亡。Western blot和RT-PCR分析显示，p21蛋白的表达上调在转录和翻译水平，由免疫组化染色支持。因此，HLSN粗毒与BMK毒相似，可通过诱导p21表达抑制HeLa细胞增殖并诱导其凋亡，而对非癌人脐静脉内皮细胞（HUVEC-21）无明显影响。
Scorpion venoms are important sources for identifying and developing promising biomolecules for cancer treatment. To search for promising biomolecules for cancer research, the effects of crude venom from the scorpion Heterometrus liangi (HLSN) on the proliferation, apoptosis and underline molecular mechanisms of HeLa cells were investigated and compared with that of Buthus martensii (BMK). RT-PCR, Western blot and immunohistochemical staining were used to evaluate the expression levels of p21 in the control and treated cells. The present data showed that when HeLa cells were exposed to HLSN venom for 24 and 48 h, the venom inhibited HeLa cell proliferation in a dose-dependent manner as determined by the methyl thiazolyl tetrazolium (MTT) assay, caused marked morphological changes as revealed by hematoxylin and eosin (HE) staining, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and induced apoptosis as shown by flow cytometry (FCM). Western blot and RT-PCR analysis revealed that the expression of p21 protein was upregulated at both transcriptional and translational levels, as supported by immunohistochemical staining. Therefore, HLSN crude venom, similar to BMK venom, inhibits proliferation and induces apoptosis in HeLa cells via the induction of p21 expression while it has little adverse effect on non cancer human umbilical vein endothelial (HUVEC-21) cells.
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DOI：
10.1159/000099415
发表时间：
2007-02
期刊：
--
影响因子：
--
作者：
J. Hulshof
通讯作者：
J. Hulshof
DOI：
10.4028/www.scientific.net/amr.345.393
发表时间：
2011-09
期刊：
Advanced Materials Research
影响因子：
--
作者：
Zhi Wang;W. Fu;Xianghuai Lu;Guang-xian Cai
通讯作者：
Zhi Wang;W. Fu;Xianghuai Lu;Guang-xian Cai
DOI：
10.1007/springerreference_15188
发表时间：
1955
期刊：
--
影响因子：
--
作者：
Frigyes Riesz
通讯作者：
Frigyes Riesz
DOI：
10.1016/0041-0101(94)90108-2
发表时间：
1994-02
期刊：
Toxicon : official journal of the International Society on Toxinology
影响因子：
--
作者：
Rui Wang;Pierre Moreau;Alain Deschamps;J. Champlain;Rémy Sauvé;Sylvain Foucart;Lin Bai;
通讯作者：
Rui Wang;Pierre Moreau;Alain Deschamps;J. Champlain;Rémy Sauvé;Sylvain Foucart;Lin Bai;
DOI：
10.4135/9781483365817.n608
发表时间：
2004
期刊：
--
影响因子：
--
作者：
Gerald Teschl
通讯作者：
Gerald Teschl