Sequential gene expression during pronephric tubule formation in vitro in Xenopus ectoderm
Sequential gene expression during pronephric tubule formation in vitro in Xenopus ectoderm
复制标题
非洲爪蟾外胚层前肾小管形成过程中的序列基因表达
DOI：
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复制
发表时间：
1996
期刊：
影响因子：
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通讯作者：
M. Asashima
中科院分区：
文献类型：
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作者：
T. Uochi;M. Asashima
肾脏已被用作分析器官发生的模型器官。在使用非洲爪蟾外胚层的体外实验中，可以通过用激活素A和视黄酸（RA）处理来诱导原肾小管（肾脏的原型）的发育。本研究探讨是否前肾小管诱导外胚层外植体表现出类似的特点，在分子水平上的正常胚胎。高频诱导（100%）的原肾小管形成所需的实验条件进行了测定，从假定的外胚层没有肌肉和脊索的发展。在诱导的原肾小管中检查了原肾标志物基因Xlim-1和Xlcaax-1的发育表达。用10 ng/mL激活素A和10−4 mol/L RA处理后，仅以高频率诱导原肾小管。诱导的原肾小管显示出与正常胚胎相同的标记基因Xlim-1和Xlcaax-1的表达时间和模式。这些结果表明，在体外发育的原肾小管诱导的推定外胚层激活素A和RA平行于正常的发展在分子水平上。
The kidney has been used as a model organ to analyze organogenesis. In in vitro experiments using Xenopus blastula ectoderm, the development of pronephric tubules (the prototype of the kidney) may be induced by treatment with activin A and retinoic acid (RA). The present study examined whether pronephric tubules induced in ectodermal explants exhibited similar characteristics to those of normal embryos at the molecular level. The experimental conditions required for high frequency induction (100%) of pronephric tubule formation from presumptive ectoderm without the development of muscle and notochord were determined. The developmental expression of the pronephros marker genes Xlim‐1 and Xlcaax‐1 was examined in induced pronephric tubules. After treatment with 10 ng/mL activin A and 10−4 mol/L RA, only pronephric tubules were induced at a high frequency. Induced pronephric tubules showed the same timing and patterns of expression for the marker genes Xlim‐1 and Xlcaax‐1 as normal embryos. These results suggest that the in vitro development of pronephric tubules induced in the presumptive ectoderm by activin A and RA parallels normal development at the molecular level.
LIM 类同源盒基因 Xlim-1 在非洲爪蟾胚胎的前肾和 CNS 细胞谱系中的表达受到视黄酸和外原肠胚形成的影响。
DOI：
10.1242/dev.120.6.1525
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
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作者：
Taira,M;Otani,H;Jamrich,M;Dawid,IB
通讯作者：
Dawid,IB
后肾间充质含有多能干细胞，其命运在诱导后受到限制。
DOI：
10.1242/dev.114.3.565
发表时间：
1992
期刊：
Development (Cambridge, England)
影响因子：
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作者：
Herzlinger,D;Koseki,C;Mikawa,T;al-Awqati,Q
通讯作者：
al-Awqati,Q
xlgv7：一种位于非洲爪蟾中枢神经系统细胞核中的母体基因产物。
影响因子：
10.5
作者：
Miller,M;Kloc,M;Reddy,B;Eastman,E;Dreyer,C;Etkin,L
通讯作者：
Etkin,L