Maternal peripheral blood natural killer cells incorporate placenta-associated microRNAs during pregnancy.
Maternal peripheral blood natural killer cells incorporate placenta-associated microRNAs during pregnancy.
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DOI：
10.3892/ijmm.2015.2157
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发表时间：
2015-06
影响因子：
5.4
通讯作者：
Takizawa T
中科院分区：
文献类型：
--
作者：
Ishida Y;Zhao D;Ohkuchi A;Kuwata T;Yoshitake H;Yuge K;Takizawa T;Matsubara S;Suzuki M;Saito S;Takizawa T
尽管最近的研究表明microRNA（miRNAs或miRs）调节基本的自然杀伤（NK）细胞过程，包括细胞毒性和细胞因子的产生，但对妊娠期间母体外周血NK（pNK）细胞中的miRNAs-基因调控关系知之甚少。在本研究中，为了确定miRNA在母体pNK细胞基因调控网络中的作用，我们使用基于逆转录定量PCR（RT-qPCR）的miRNA阵列和DNA微阵列分析的组合对母体pNK细胞进行了全面的miRNA和基因表达谱分析，并分析了孕早期和孕晚期pNK细胞之间的差异表达水平。此外，我们构建了调控网络的miRNA介导的基因表达的pNK细胞在怀孕期间的免疫途径分析（IPA）。基于PCR的阵列分析显示，在妊娠期间在pNK细胞中检测到胎盘来源的miRNA [染色体19 miRNA簇（C19 MC）miRNA]。25种miRNA，包括6种C19 MC miRNA，在晚期与早期妊娠pNK细胞相比显著上调。在递送后，在pNK细胞中也发生C19 MC miRNA的快速清除。9个miRNA，包括8个C19 MC miRNA，在分娩后的pNK细胞中与妊娠晚期相比显著下调。DNA微阵列分析确定了69个NK细胞功能相关基因，这些基因在孕早期和孕晚期pNK细胞之间差异表达。在途径和网络分析中，观察到的pNK细胞的基因表达变化可能有助于细胞毒性的增加，以及与妊娠早期相比，妊娠晚期pNK细胞的细胞周期进展。在69个NK细胞功能相关基因中，有13个在孕早期和孕晚期的pNK细胞中显著下调。13个下调的NK功能相关基因中的9个是12个上调的miRNA的计算机模拟靶候选物，包括C19 MC miRNA miR-512- 3 p。这项研究的结果表明，胎盘C19 MC miRNA转移到母体pNK细胞发生在怀孕期间。目前的研究提供了对母体NK细胞功能的新见解。
Although recent studies have demonstrated that microRNAs (miRNAs or miRs) regulate fundamental natural killer (NK) cellular processes, including cytotoxicity and cytokine production, little is known about the miRNA-gene regulatory relationships in maternal peripheral blood NK (pNK) cells during pregnancy. In the present study, to determine the roles of miRNAs within gene regulatory networks of maternal pNK cells, we performed comprehensive miRNA and gene expression profiling of maternal pNK cells using a combination of reverse transcription quantitative PCR (RT-qPCR)-based miRNA array and DNA microarray analyses and analyzed the differential expression levels between first- and third-trimester pNK cells. Furthermore, we constructed regulatory networks for miRNA-mediated gene expression in pNK cells during pregnancy by Ingenuity Pathway Analysis (IPA). PCR-based array analysis revealed that the placenta-derived miRNAs [chromosome 19 miRNA cluster (C19MC) miRNAs] were detected in pNK cells during pregnancy. Twenty-five miRNAs, including six C19MC miRNAs, were significantly upregulated in the third- compared to first-trimester pNK cells. The rapid clearance of C19MC miRNAs also occurred in the pNK cells following delivery. Nine miRNAs, including eight C19MC miRNAs, were significantly downregulated in the post-delivery pNK cells compared to those of the third-trimester. DNA microarray analysis identified 69 NK cell function-related genes that were differentially expressed between the first- and third-trimester pNK cells. On pathway and network analysis, the observed gene expression changes of pNK cells likely contribute to the increase in the cytotoxicity, as well as the cell cycle progression of third- compared to first-trimester pNK cells. Thirteen of the 69 NK cell function-related genes were significantly down-regulated between the first- and third-trimester pNK cells. Nine of the 13 downregulated NK-function-associated genes were in silico target candidates of 12 upregulated miRNAs, including C19MC miRNA miR-512-3p. The results of this study suggest that the transfer of placental C19MC miRNAs into maternal pNK cells occurs during pregnancy. The present study provides new insight into maternal NK cell functions.
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影响因子：
4.4
作者：
Dybkaer K;Iqbal J;Zhou G;Geng H;Xiao L;Schmitz A;d'Amore F;Chan WC
通讯作者：
Chan WC
影响因子：
30.8
作者：
Bentwich, I;Avniel, A;Bentwich, Z
通讯作者：
Bentwich, Z
DOI：
10.1084/jem.20100570
发表时间：
2010-09-27
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Joncker NT;Shifrin N;Delebecque F;Raulet DH
通讯作者：
Raulet DH
影响因子：
3.7
作者：
Allantaz F;Cheng DT;Bergauer T;Ravindran P;Rossier MF;Ebeling M;Badi L;Reis B;Bitter H;D'Asaro M;Chiappe A;Sridhar S;Pacheco GD;Burczynski ME;Hochstrasser D;Vonderscher J;Matthes T
通讯作者：
Matthes T
影响因子：
4.4
作者：
Hedlund, Malin;Stenqvist, Ann-Christin;Mincheva-Nilsson, Lucia
通讯作者：
Mincheva-Nilsson, Lucia