Rapid Resetting of the Mammalian Circadian Clock
Rapid Resetting of the Mammalian Circadian Clock
复制标题
哺乳动物昼夜节律时钟的快速重置
DOI：
10.1523/jneurosci.19-02-00828.1999
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
M. Hastings
中科院分区：
文献类型：
--
作者：
J. D. Best;E. Maywood;Karen L. Smith;M. Hastings
视交叉上核（SCN）包含主要的昼夜节律时钟控制明显的生理和行为的日常节奏。内源性昼夜周期通过直接谷氨酸能视网膜传入到SCN的光/暗传递。为了理解夹带的分子基础，首先有必要确定光脉冲重置时钟的速度。我们使用双脉冲模式，结合SCN功能的细胞和行为分析，探索叙利亚仓鼠和小鼠昼夜节律振荡器的重置速度。对c-fos诱导和cAMP反应元件结合蛋白磷酸化的分析表明，SCN能够分辨并响应间隔1或2小时的光脉冲。对单脉冲或双脉冲仓鼠的昼夜轮运动节律的相移分析表明，振荡器在2小时内发生重置。这是延迟和提前相移的情况。对小鼠延迟移位的检查显示在2小时内重置，此外还显示在光脉冲的1小时内未完成重置。这些结果建立了一个时间窗口，在其中定义哺乳动物昼夜节律重置的主要分子机制。
The suprachiasmatic nuclei (SCN) contain the principal circadian clock governing overt daily rhythms of physiology and behavior. The endogenous circadian cycle is entrained to the light/dark via direct glutamatergic retinal afferents to the SCN. To understand the molecular basis of entrainment, it is first necessary to define how rapidly the clock is reset by a light pulse. We used a two-pulse paradigm, in combination with cellular and behavioral analyses of SCN function, to explore the speed of resetting of the circadian oscillator in Syrian hamster and mouse. Analysis of c-fos induction and cAMP response element-binding protein phosphorylation in the retinorecipient SCN demonstrated that the SCN are able to resolve and respond to light pulses presented 1 or 2 hr apart. Analysis of the phase shifts of the circadian wheel-running activity rhythm of hamsters presented with single or double pulses demonstrated that resetting of the oscillator occurred within 2 hr. This was the case for both delaying and advancing phase shifts. Examination of delaying shifts in the mouse showed resetting within 2 hr and in addition showed that resetting is not completed within 1 hr of a light pulse. These results establish the temporal window within which to define the primary molecular mechanisms of circadian resetting in the mammal.
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影响因子：
56.9
作者：
Plautz, JD;Kaneko, M;Kay, SA
通讯作者：
Kay, SA
光对人体昼夜节律起搏器的影响。
DOI：
10.1002/9780470514597.ch14
发表时间：
1995
期刊：
Ciba Foundation symposium
影响因子：
--
作者：
Czeisler,CA
通讯作者：
Czeisler,CA
影响因子：
56.9
作者：
GINTY, DD;KORNHAUSER, JM;GREENBERG, ME
通讯作者：
GREENBERG, ME
影响因子：
5.5
作者：
NELSON, DE;TAKAHASHI, JS
通讯作者：
TAKAHASHI, JS