Spatial models of cell distribution in human lumbar dorsal root ganglia.
Spatial models of cell distribution in human lumbar dorsal root ganglia.
复制标题
人腰椎背根神经节细胞分布的空间模型。
DOI：
10.1002/cne.24848
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Bruns,TimM
中科院分区：
文献类型：
--
作者：
Sperry,ZachariahJ;Graham,RobertD;Peck-Dimit,Nicholas;Lempka,ScottF;Bruns,TimM
背根神经节（Dorsal root ganglia， DRG）包含初级感觉神经元的体体，越来越多地被认为是临床神经接口的新靶点，无论是在神经修复还是疼痛应用中。有效地使用神经记录或刺激技术需要相对于目标神经元件（无论是轴突还是细胞体）的适当空间位置。然而，人类DRG神经纤维和躯体的内部三维空间组织尚未被定量描述。在这项研究中，我们分析了来自10个供体的31个L4和L5 DRG的202张横断面图像。我们使用自定义的半自动图形用户界面来识别图像中神经元素的位置，并将输出归一化为一致的空间参考，以便通过脊柱水平进行直接比较。通过应用递归划分算法，我们发现两个脊柱水平的细胞体密度最高的地方是DRG长度的内85%，径向上最多25-30%，背侧最多69-76%。轴突密度沿DRG长度分布均匀，但沿径向向外7-11%存在明显的低密度区。这些发现与之前关于DRG神经分布的定性报告一致。我们提供的定量测量将使未来神经接口技术和以DRG为重点的药物治疗的靶向性得到改善，并为中枢和周围神经系统之间的桥梁提供严格的解剖学描述。
Dorsal root ganglia (DRG), which contain the somata of primary sensory neurons, have increasingly been considered as novel targets for clinical neural interfaces, both for neuroprosthetic and pain applications. Effective use of either neural recording or stimulation technologies requires an appropriate spatial position relative to the target neural element, whether axon or cell body. However, the internal three‐dimensional spatial organization of human DRG neural fibers and somata has not been quantitatively described. In this study, we analyzed 202 cross‐sectional images across the length of 31 human L4 and L5 DRG from 10 donors. We used a custom semi‐automated graphical user interface to identify the locations of neural elements in the images and normalize the output to a consistent spatial reference for direct comparison by spinal level. By applying a recursive partitioning algorithm, we found that the highest density of cell bodies at both spinal levels could be found in the inner 85% of DRG length, the outer‐most 25–30% radially, and the dorsal‐most 69–76%. While axonal density was fairly homogeneous across the DRG length, there was a distinct low density region in the outer 7–11% radially. These findings are consistent with previous qualitative reports of neural distribution in DRG. The quantitative measurements we provide will enable improved targeting of future neural interface technologies and DRG‐focused pharmaceutical therapies, and provide a rigorous anatomical description of the bridge between the central and peripheral nervous systems.
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影响因子：
2.5
作者：
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DOI：
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