Arginine inhibits adsorption of proteins on polystyrene surface.
Arginine inhibits adsorption of proteins on polystyrene surface.
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DOI：
10.1371/journal.pone.0070762
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shiraki K
中科院分区：
文献类型：
--
作者：
Shikiya Y;Tomita S;Arakawa T;Shiraki K
蛋白质在固体表面的非特异性吸附会导致提纯和储存过程中蛋白质浓度的降低和酶的失活。然而，在低浓度下，没有通用的抑制剂来抑制蛋白质与固体表面之间的吸附。因此，我们研究了防止蛋白质吸附在聚苯乙烯颗粒(PS颗粒)上的添加剂，作为一次性试管和微管等容器的常用材料。将蛋白质溶液与PS颗粒混合，然后通过离心后上清液中蛋白质的浓度和活性来监测蛋白质的吸附。五种不同的蛋白质通过静电、疏水和芳香相互作用与PS颗粒结合，导致蛋白质浓度下降和上清液中酶活性丧失。在精氨酸盐酸盐(Arg)、赖氨酸盐酸盐、盐酸胍、氯化钠、甘氨酸和葡萄糖等添加剂中，Arg在防止蛋白质与PS颗粒结合和活性丧失方面效果最好。此外，即使在蛋白质和PS颗粒混合后，Arg的加入也会导致结合蛋白从PS颗粒上解吸。本研究展示了Arg的一种新功能，从而扩展了Arg在蛋白质中的应用潜力。
Nonspecific adsorption of protein on solid surfaces causes a reduction of concentration as well as enzyme inactivation during purification and storage. However, there are no versatile inhibitors of the adsorption between proteins and solid surfaces at low concentrations. Therefore, we examined additives for the prevention of protein adsorption on polystyrene particles (PS particles) as a commonly-used material for vessels such as disposable test tubes and microtubes. A protein solution was mixed with PS particles, and then adsorption of protein was monitored by the concentration and activity of protein in the supernatant after centrifugation. Five different proteins bound to PS particles through electrostatic, hydrophobic, and aromatic interactions, causing a decrease in protein concentration and loss of enzyme activity in the supernatant. Among the additives, including arginine hydrochloride (Arg), lysine hydrochloride, guanidine hydrochloride, NaCl, glycine, and glucose, Arg was most effective in preventing the binding of proteins to PS particles as well as activity loss. Moreover, even after the mixing of protein and PS particles, the addition of Arg caused desorption of the bound protein from PS particles. This study demonstrated a new function of Arg, which expands the potential for application of Arg to proteins.
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影响因子：
2.9
作者：
KITA, Y;ARAKAWA, T;TIMASHEFF, SN
通讯作者：
TIMASHEFF, SN
影响因子：
--
作者：
Arakawa, Tsutomu;Tsumoto, Kouhei;Tokunaga, Masao
通讯作者：
Tokunaga, Masao
影响因子：
1.6
作者：
Arakawa, Tsutomu;Futatsumori-Sugai, Mutsumi;Ejima, Daisuke
通讯作者：
Ejima, Daisuke
影响因子：
3.9
作者：
Evers, Florian;Steitz, Roland;Czeslik, Claus
通讯作者：
Czeslik, Claus
影响因子：
2.9
作者：
Ejima, D;Yumioka, R;Arakawa, T
通讯作者：
Arakawa, T