Differential localization of organic cation transporters rOCT1 and rOCT2 in the basolateral membrane of rat kidney proximal tubules
Differential localization of organic cation transporters rOCT1 and rOCT2 in the basolateral membrane of rat kidney proximal tubules
复制标题
有机阳离子转运蛋白rOCT1和rOCT2在大鼠肾近曲小管基底外侧膜中的差异定位
DOI：
10.1007/s004180000186
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发表时间：
2000
影响因子：
2.3
通讯作者：
K. Takata
中科院分区：
文献类型：
--
作者：
M. Sugawara;Y. Urakami;H. Koyama;K. Fujikura;S. Masuda;H. Saito;T. Naruse;K. Inui;K. Takata
抽象。有机阳离子转运蛋白在肾小管分泌阳离子药物和内源性阳离子代谢产物中起重要作用。免疫印迹显示，在大鼠肾脏中存在有机阳离子转运蛋白，rOCT 1和rOCT 2。通过免疫荧光显微镜，rOCT 1被证明集中在肾皮质的近端小管。另一方面，rOCT 2在外髓质外条的近端小管中丰富。共聚焦显微镜显示rOCT 1和rOCT 2均定位于这些小管细胞的基底外侧膜。这些发现直接表明，rOCT 1和rOCT 2是基底外侧膜蛋白，并沿近端小管沿着差异分布。
Abstract. Organic cation transporters play an important role in the secretion of cationic drugs as well as endogenous cationic metabolites in the renal tubules. Immunoblotting showed the presence of organic cation transporter proteins, rOCT1 and rOCT2, in the rat kidney. By immunofluorescence microscopy, rOCT1 was shown to be concentrated in the proximal tubules in the renal cortex. rOCT2, on the other hand, was rich in the proximal tubules in the outer stripe of the outer medulla. Confocal microscopy revealed that both rOCT1 and rOCT2 were localized to the basolateral membranes of these tubule cells. These findings directly show that rOCT1 and rOCT2 are basolateral membrane proteins and are differentially distributed along the proximal tubules.
DOI：
10.1152/ajpcell.1990.259.2.c279
发表时间：
1990-07
期刊：
The American journal of physiology
影响因子：
--
作者：
B. Thorens;Z. Cheng;Dennis Brown;H. Lodish
通讯作者：
B. Thorens;Z. Cheng;Dennis Brown;H. Lodish