Protein adsorption and cell adhesion on nanoscale bioactive coatings formed from poly(ethylene glycol) and albumin microgels.
Protein adsorption and cell adhesion on nanoscale bioactive coatings formed from poly(ethylene glycol) and albumin microgels.
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DOI：
10.1016/j.biomaterials.2008.08.003
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发表时间：
2008-12
期刊：
影响因子：
14
通讯作者：
Elbert, Donald L.
中科院分区：
文献类型：
--
作者：
Scott, Evan A.;Nichols, Michael D.;Cordova, Lee H.;George, Brandon J.;Jun, Young-Shin;Elbert, Donald L.
药物洗脱支架上的晚期血栓形成是一个新出现的问题，可以使用极薄的生物活性水凝胶涂层来解决。我们报道了一种将聚乙二醇（PEG）共价连接到基底上的浸涂策略，产生厚度<100 nm的涂层。通过动态光散射（DLS）监测PEG-八乙烯砜与牛血清白蛋白（BSA）或PEG-八胺中的胺的凝胶化，揭示在宏观凝胶化之前存在微凝胶。NMR还揭示了极高的端基转化之前，大凝胶化，形成高度交联的微凝胶和偏离Flory-Stockmayer理论一致。在宏观凝胶化之前，将反应溶液稀释并与亲核试剂官能化的表面一起孵育。使用光波导光模光谱（OWLS）和石英晶体微天平与耗散（QCM-D），我们确定了一个高度水合，抗蛋白质层的厚度约为75 nm。在缓冲水中的原子力显微镜显示存在各种尺寸的聚结球，但直径小于约100 nm。微凝胶涂层玻璃或聚（对苯二甲酸乙二醇酯）表现出减少蛋白质吸附和细胞粘附。细胞与表面的相互作用可以通过使用不同的蛋白质来覆盖涂层内未反应的乙烯砜基团来控制。
Late-term thrombosis on drug-eluting stents is an emerging problem that might be addressed using extremely thin, biologically-active hydrogel coatings. We report a dip-coating strategy to covalently link poly(ethylene glycol) (PEG) to substrates, producing coatings with <≈100 nm thickness. Gelation of PEG-octavinylsulfone with amines in either bovine serum albumin (BSA) or PEG-octaamine was monitored by dynamic light scattering (DLS), revealing the presence of microgels before macrogelation. NMR also revealed extremely high end group conversions prior to macrogelation, consistent with the formation of highly crosslinked microgels and deviation from Flory-Stockmayer theory. Before macrogelation, the reacting solutions were diluted and incubated with nucleophile-functionalized surfaces. Using optical waveguide lightmode spectroscopy (OWLS) and quartz crystal microbalance with dissipation (QCM-D), we identified a highly hydrated, protein-resistant layer with a thickness of approximately 75 nm. Atomic force microscopy in buffered water revealed the presence of coalesced spheres of various sizes but with diameters less than about 100 nm. Microgel-coated glass or poly(ethylene terephthalate) exhibited reduced protein adsorption and cell adhesion. Cellular interactions with the surface could be controlled by using different proteins to cap unreacted vinylsulfone groups within the coating.
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影响因子：
6.2
作者：
Elbert, DL;Hubbell, JA
通讯作者：
Hubbell, JA
影响因子：
5.5
作者：
Gan, DJ;Lyon, LA
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Lyon, LA
影响因子：
5.5
作者：
Ghosh, P;Lackowski, WM;Crooks, RM
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Crooks, RM
影响因子：
1.8
作者：
Graham, NB;Cameron, A
通讯作者：
Cameron, A
影响因子：
15
作者：
Flory, PJ
通讯作者：
Flory, PJ