Validation of Potential Reference Genes for Real-Time qPCR Analysis in Pharaoh Ant, Monomorium pharaonis (Hymenoptera: Formicidae).
Validation of Potential Reference Genes for Real-Time qPCR Analysis in Pharaoh Ant, Monomorium pharaonis (Hymenoptera: Formicidae).
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验证法老蚁、Monomorium pharaonis（膜翅目：蚁科）实时 qPCR 分析的潜在参考基因
DOI：
10.3389/fphys.2022.852357
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发表时间：
2022
影响因子：
4
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Ding G;Gao Q;Chen J;Zhao J;Zhang G;Liu W
蚂蚁是高度多样化的社会性昆虫，生活在由数百万个体组成的群体中，具有生殖分工。由于对揭示蚁群中不同等级和分工的遗传和表观遗传调控机制的兴趣，许多蚂蚁物种最近被建立为进化发展和社会行为研究的实验室模型。这些功能研究通常要求精确定量相对基因表达水平，这依赖于稳定表达的参考基因进行归一化。然而，在蚂蚁中还没有建立起一套用于此目的的核心可靠参考基因。在本研究中，我们测试了12个丰富表达的候选基因的表达模式和扩增效率Monomorium pharaonis，少数几个蚂蚁物种之一，是适合实验室饲养和实验。我们通过RT-qPCR定量了这些基因在七种不同条件下的表达水平：胚胎发育、性发育、工蜂发育、成虫表型、组织和两种非生物操纵处理。最后，五个基因，延伸因子-1 α（EF 1A），甘油醛3-磷酸脱氢酶（GAPDH），TATA盒结合蛋白（TATA），微管蛋白γ-2链样（TBLg 2），热休克蛋白67 B2样（HSP 67）被认为是在七种条件下最稳定的参考基因。我们还确定了适用于每种不同条件的最稳定的参考基因，并评估了所需参考基因的最佳数量。本研究为RT-qPCR分析提供了可靠的参考基因，为进一步的药物研究奠定了基础。
Ants are highly diverse social insects living in colonies consisted of up to millions of individuals with reproductive division of labors. Due to the interests in disclosing the genetic and epigenetic regulation mechanisms underlying the distinct developmental trajectories between castes and division of labor in colonies, many ant species have recently been established as laboratory models for evolutionary development and social behavior studies. These functional studies often request a precise quantification of the relative gene expression level, which relies on a stably expressed reference genes for normalization. A core set of reliable reference genes for this purpose however has not been established yet in ants. In the present study, we tested the expression patterns and amplification efficiencies of 12 abundantly expressed candidate genes in Monomorium pharaonis, one of the few ant species that are suitable for laboratory rearing and experimentation. We quantified the expression levels of these genes by RT-qPCR in seven different conditions: embryo development, sexual development, worker development, adult phenotypes, tissues, and two abiotic manipulative treatments in pharaoh ant. Finally, five genes, elongation factor-1 alpha (EF1A), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), TATA-box-binding protein (TATA), tubulin gamma-2 chain-like (TBLg2), heat shock protein 67B2-like (HSP67) were found to be the most stable reference genes across seven conditions. We also identified the most stable reference genes applicable for each distinct condition and the optimal number of reference genes entailed were evaluated. Our study validates reliable reference genes for RT-qPCR analysis which lays the foundation for future studies in pharaoh ant.
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切叶蚁 Acromyrmex echinatior 中种姓特异性 RNA 编辑组
影响因子：
16.6
作者：
Li, Qiye;Wang, Zongji;Lian, Jinmin;Schiott, Morten;Jin, Lijun;Zhang, Pei;Zhang, Yanyan;Nygaard, Sanne;Peng, Zhiyu;Zhou, Yang;Deng, Yuan;Zhang, Wenwei;Boomsma, Jacobus J.;Zhang, Guojie
通讯作者：
Zhang, Guojie
DOI：
10.1126/science.aar5723
发表时间：
2018-07-27
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
Chandra V;Fetter-Pruneda I;Oxley PR;Ritger AL;McKenzie SK;Libbrecht R;Kronauer DJC
通讯作者：
Kronauer DJC
影响因子：
4
作者：
Lü J;Yang C;Zhang Y;Pan H
通讯作者：
Pan H
DOI：
10.1073/pnas.96.10.5575
发表时间：
1999-05-11
影响因子：
11.1
作者：
Evans, JD;Wheeler, DE
通讯作者：
Wheeler, DE
影响因子：
56.9
作者：
Abouheif, E;Wray, GA
通讯作者：
Wray, GA