Genome Size of 17 Species From Caelifera (Orthoptera) and Determination of Internal Standards With Very Large Genome Size in Insecta.
Genome Size of 17 Species From Caelifera (Orthoptera) and Determination of Internal Standards With Very Large Genome Size in Insecta.
复制标题
昆虫纲 17 个直翅目物种的基因组大小和基因组非常大的内标测定
DOI：
10.3389/fphys.2020.567125
复制
发表时间：
2020
影响因子：
4
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Mao Y;Zhang N;Nie Y;Zhang X;Li X;Huang Y
对昆虫基因组大小的比较研究表明，直翅目是昆虫纲中一个独特的类群，基因组明显增大。为了确定一个合适的内标一个大的基因组，并比较不同的内标对基因组大小的估计的影响，我们使用了四个内标，以估计核DNA含量在9个昆虫物种的大基因组。结果表明，飞蝗（Locusta migratoria）和美洲大蠊（Periplaneta americana）两种内标物（1C = 6.20 pg，1C = 6.60 pg，1C = 3.41 pg）的组合适合于利用流式细胞术进行大基因组的检测。利用这两个内标，我们用流式细胞术估计了17种Caelifera（蝗科12属，癞蝗科2属，锥头蝗科1属）的基因组大小。基因组范围从6.57 pg（Shirakiacris shirakii）到18.64 pg（Bryodemella holdereri），是迄今为止在昆虫中描述的最大的。这些物种的基因组在性别上表现出明显的二态性：雌性比雄性平均大0.56 pg，这可能是由于X 0（X1）/XX（X2）的性染色体决定机制。为了测试通过流式细胞术获得的结果，我们使用Illumina测序数据的k聚体来测量Calliptamus damatus和Haplotropis brunneriana的C值。两种方法的结果略有不同。估计基因组分别比流式细胞术值小约0.28和0.26 pg。此外，我们还重建了这些类群的进化关系，并讨论了基因组大小的进化在系统发育框架。
Comparative studies of insect genome size show that Orthoptera is a unique group of Insecta with a significantly enlarged genome. To determine a suitable internal standard for a large genome and to compare the effects of different internal standards on estimates of genome size, we used four internal standards to estimate nuclear DNA content in nine insect species with large genomes. The results showed that the combination of two internal standards, Locusta migratoria (♂1C = 6.20 pg, ♀1C = 6.60 pg) and Periplaneta americana♂ (1C = 3.41 pg), was suitable for estimating large genome of Caelifera by flow cytometry. Using these two internal standards, we estimated the genome sizes of 17 species of Caelifera (12 genera in Acrididae, 2 genera in Pamphagidae, 1 genus in Pyrgomorphidae) using flow cytometry. Genomes ranged from 6.57 pg (Shirakiacris shirakii) to 18.64 pg (Bryodemella holdereri), the largest described in insects to date. These species showed significant genomic dimorphism based on sex: females had a 0.56 pg larger genome than males on average, which might be due to the sex chromosome determinism mechanism of X0(♂)/XX(♀). To test the results obtained by flow cytometry, we used k-mers of Illumina sequencing data to gauge the C-value of Calliptamus abbreviatus and Haplotropis brunneriana. The results of the two methods are slightly different. Genomes were estimated to be about 0.28 and 0.26 pg smaller, respectively, than the flow cytometry values. Furthermore, we also reconstructed the evolutionary relationships of these taxa and discuss the genome size evolution in a phylogenetic framework.
登录
查看更多内容
影响因子：
1.9
作者：
Brown, J. K.;Lambert, G. M.;Galbraith, D. W.
通讯作者：
Galbraith, D. W.
影响因子：
4.2
作者：
Dolezel, J;Greilhuber, J;Obermayer, R
通讯作者：
Obermayer, R
影响因子：
1.5
作者：
Castillo, Elio R. D.;Bidau, Claudio J.;Marti, Dardo A.
通讯作者：
Marti, Dardo A.
影响因子：
4.2
作者：
Dolezel, J;Bartos, J
通讯作者：
Bartos, J
影响因子：
1.6
作者：
Camacho, J. P. M.;Ruiz-Ruano, F. J.;Bakkali, M.
通讯作者：
Bakkali, M.