Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity.
Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity.
复制标题
DOI：
10.1136/thx.2009.116061
复制
发表时间：
2010-03
期刊：
影响因子：
10
通讯作者：
Kotecha S
中科院分区：
文献类型：
--
作者：
Davies PL;Spiller OB;Beeton ML;Maxwell NC;Remold-O'Donnell E;Kotecha S
蛋白水解失衡与“经典”早产儿慢性肺病（CLD）的发生有关。然而，在“新”CLD中，这种模式发生了变化。本研究探讨了通气早产儿中性粒细胞蛋白酶及其抑制剂之间的纵向关系及其与微生物定植的关系。系列支气管肺泡灌洗液来自通气的新生早产儿。测定细胞数、神经弹性蛋白酶（NE）活性、金属蛋白酶（MMP）9、MMP 9/TIMP 1复合物、SerpinB 1浓度及SerpinB 1和α1-抗胰蛋白酶（AAT）与弹性蛋白酶复合物的百分比。使用16 S rRNA基因的PCR检查微生物基因的存在。与呼吸窘迫综合征（RDS）缓解的婴儿（2/17）相比，发生CLD的婴儿至少有一个样本（10/20）具有NE活性。然而，NE活性存在于少数样品中，作为情节性峰值发生。MMP-9、MMP-9/TIMP-1复合物峰值水平、复合物中AAT和SerpinB 1的百分比以及细胞计数在CLD婴儿中均显著高于RDS婴儿。峰值经常发生的情节尖峰和强烈的时间关系，注意到所有标记物之间。所有变量的峰值彼此显著相关。细菌16 S rRNA基因的存在与CLD的发展以及弹性蛋白酶和MMP-9的升高相关。NE活性和MMP-9似乎是重要的“新的”CLD的发展与蛋白酶和抑制剂的浓度增加episodically，可能在出生后感染。
A proteolytic imbalance has been implicated in the development of “classical” chronic lung disease of prematurity (CLD). However, in “new” CLD this pattern has changed. This study examines the longitudinal relationship between neutrophil proteinases and their inhibitors in ventilated preterm infants and their relationship to microbial colonisation. Serial bronchoalveolar lavage fluid was obtained from ventilated newborn preterm infants. Neutrophil elastase (NE) activity, cell counts, metalloproteinase (MMP)-9, MMP-9/TIMP-1 complex, SerpinB1 concentration and percentage of SerpinB1 and α1-antitrypsin (AAT) in complex with elastase were measured. The presence of microbial genes was examined using PCR for 16S rRNA genes. Statistically more infants who developed CLD had NE activity in at least one sample (10/20) compared with infants with resolved respiratory distress syndrome (RDS) (2/17). However, NE activity was present in a minority of samples, occurring as episodic peaks. Peak levels of MMP-9, MMP-9/TIMP-1 complex, percentage of AAT and SerpinB1 in complex and cell counts were all statistically greater in infants developing CLD than in infants with resolved RDS. Peak values frequently occurred as episodic spikes and strong temporal relationships were noted between all markers. The peak values for all variables were significantly correlated to each other. The presence of bacterial 16S rRNA genes was associated with the development of CLD and with elevated elastase and MMP-9. NE activity and MMP-9 appear to be important in the development of “new” CLD with both proteinase and inhibitor concentrations increasing episodically, possibly in response to postnatal infection.
登录
查看更多内容
影响因子：
9.4
作者：
Davies, CE;Hill, KE;Thomas, DW
通讯作者：
Thomas, DW
影响因子：
8
作者：
Ekekezie, II;Thibeault, DW;Truog, WE
通讯作者：
Truog, WE
影响因子：
3.6
作者：
Kotecha, S;Wilson, L;Shaw, RJ
通讯作者：
Shaw, RJ
影响因子：
3.6
作者：
Miralles, R;Hodge, R;Kotecha, S
通讯作者：
Kotecha, S
影响因子：
15.9
作者：
MERRITT, TA;COCHRANE, CG;GLUCK, L
通讯作者：
GLUCK, L