Evaluation of immune responses induced by a novel human papillomavirus type 16 E7 peptide-based vaccine with Candida skin test reagent as an adjuvant in C57BL/6 mice.
Evaluation of immune responses induced by a novel human papillomavirus type 16 E7 peptide-based vaccine with Candida skin test reagent as an adjuvant in C57BL/6 mice.
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新型人乳头瘤病毒 16 型 E7 肽疫苗诱导的免疫反应评估
DOI：
10.1016/j.intimp.2018.01.037
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发表时间：
2018-03
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Wang X;Che Y;Chen B;Zhang Y;Nakagawa M;Wang X
细胞免疫(CMI)反应对于清除人乳头瘤病毒(HPV)感染和HPV相关病变至关重要。活化的CD8T细胞是识别和杀伤HPV感染或HPV转化细胞的关键效应细胞。CD4T细胞为CD8T细胞的产生和维持以及肿瘤免疫提供了帮助。一种理想的治疗性HPV多肽疫苗应同时诱导强大的CD8T细胞反应和CD4T细胞反应，以确保其有效性。本课题组在体外实验中证实念珠菌皮试试剂能诱导朗格汉斯细胞分泌IL-12和T细胞增殖，表明念珠菌具有增强CMI应答的潜力。在本研究中，我们设计了一种新型的HPV多肽疫苗，该疫苗包括HPV16E7多肽和假丝酵母菌作为佐剂。对疫苗诱导的免疫应答进行综合评价。结果表明，该疫苗诱导了显著的HPV特异性CD8T细胞和Th1CD4T细胞应答以及体液免疫应答。有趣的是，念珠菌单独诱导Th1应答和干扰素-ɣ的产生显著极化，这表明念珠菌不仅可以作为一种潜在的免疫治疗试剂用于治疗HPVs相关的病变，而且还可以用于其他病毒感染甚至癌症。
Cell mediated immune (CMI) responses are crucial for the clearance of human papillomavirus (HPV) infection and HPV-associated lesions. Activated CD8 T cells are critical effector cells in recognizing and killing HPV-infected or HPV-transformed cells. CD4 T cells provide help for priming the generation and maintenance of CD8 T cells as well as for tumors immunity. An ideal therapeutic HPV peptide-based vaccines should induce both a robust CD8 T-cell response as well as a CD4 T-cell response for ensuring their efficiency. Candida skin test reagent was demonstrated to be able to induce the secretion of IL-12 by Langerhans cells and T-cell proliferation in vitro by our group, which indicated the potential of Candida to enhance CMI response. In this current study, we designed a novel HPV peptide-based vaccine which includes HPV16 E7 peptides and Candida as an adjuvant. The immune response induced by the vaccine was comprehensively evaluated. The results showed that the vaccine induced significant HPV-specific CD8 T-cell and Th1 CD4 T-cell responses as well as humoral immune response. It is interesting that Candida alone induced significant polarization of Th1 response and the production of IFN-ɣ, which indicated Candida alone may be used as a potential immunotherapeutic reagent not only for HPV-associated lesions but also for other viral infection or even cancers.
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影响因子：
20.3
作者：
Hilkens, CMU;Kalinski, P;Kapsenberg, ML
通讯作者：
Kapsenberg, ML
DOI：
10.1007/s00262-016-1821-x
发表时间：
2016-05
期刊：
Cancer immunology, immunotherapy : CII
影响因子：
--
作者：
Coleman HN;Greenfield WW;Stratton SL;Vaughn R;Kieber A;Moerman-Herzog AM;Spencer HJ;Hitt WC;Quick CM;Hutchins LF;Mackintosh SG;Edmondson RD;Erickson SW;Nakagawa M
通讯作者：
Nakagawa M
影响因子：
5.5
作者：
Wang, Xuelian;Coleman, Hannah N.;Nagarajan, Uma;Spencer, Horace J.;Nakagawa, Mayumi
通讯作者：
Nakagawa, Mayumi
DOI：
10.1111/igc.0b013e3181a388c4
发表时间：
2009-05
期刊：
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society
影响因子：
--
作者：
Farhat S;Nakagawa M;Moscicki AB
通讯作者：
Moscicki AB
影响因子：
0.5
作者：
Denny, Lynette
通讯作者：
Denny, Lynette