Knockdown of HIP1 expression promotes ligand‑induced endocytosis of EGFR in HeLa cells.
Knockdown of HIP1 expression promotes ligand‑induced endocytosis of EGFR in HeLa cells.
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HIP1表达的敲低促进了HeLa细胞中配体诱导的EGFR内吞作用。
DOI：
10.3892/or.2017.6025
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发表时间：
2017-12
期刊：
影响因子：
4.2
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Li D;Chen F;Ding J;Lin N;Li Z;Wang X
亨廷顿相互作用蛋白1（HIP 1）与多种肿瘤类型相关;然而，其在肿瘤细胞中的确切功能尚不清楚。在这项研究中，HIP 1对EGFR降解的影响，这在EGF刺激后的致癌作用中具有重要作用。筛选17株细胞系后，在HeLa细胞中检测到HIP 1和EGFR的共表达。因此，使用各种HIP 1 siRNA序列在HeLa细胞中敲低HIP 1的表达。在用各种浓度的EGF刺激后，检测HeLa细胞中EGFR的内吞作用和网格蛋白的定位（即，1.5和100 ng/ml）。在HIP 1表达被siRNA阻断后，EGFR的内吞作用被加速，并且这种作用依赖于EGF的浓度。这种内吞作用与网格蛋白表达共定位。这些结果表明，HIP 1的抑制可以加速EGFR的内吞和降解。此外，他们认为HIP 1是各种癌症类型的潜在治疗靶点，特别是那些EGFR高表达的癌症，但需要进一步研究来验证这一假设。
Huntington-interacting protein 1 (HIP1) is associated with various tumor types; however, its precise functions in tumor cells are unclear. In this study, the effects of HIP1 on the degradation of EGFR, which have important roles in carcinogenesis after EGF stimulation, were examined. After screening 17 cell lines, the coexpression of HIP1 and EGFR was detected in HeLa cells. Accordingly, the expression of HIP1 was knocked down in HeLa cells using various HIP1 siRNA sequences. The endocytosis of EGFR and localization of clathrin in HeLa cells were examined after stimulation by EGF at various concentrations (i.e., 1.5 and 100 ng/ml). After HIP1 expression was blocked by siRNAs, EGFR endocytosis was accelerated and this effect was dependent on the EGF concentration. This endocytosis was colocalized with clathrin expression. These findings indicate that the inhibition of HIP1 can accelerate the endocytosis and degradation of EGFR. Furthermore, they suggest that HIP1 is a potential therapeutic target for various cancer types, particularly those with high EGFR expression, but further research is needed to examine this hypothesis.
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影响因子：
11.2
作者：
Bradley, Sarah V.;Holland, Eric C.;Ross, Theodora S.
通讯作者：
Ross, Theodora S.
DOI：
10.1111/tra.12165
发表时间：
2014-06
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Mutch LJ;Howden JD;Jenner EP;Poulter NS;Rappoport JZ
通讯作者：
Rappoport JZ
影响因子：
50.3
作者：
Rao, DS;Bradley, SV;Ross, TS
通讯作者：
Ross, TS
影响因子：
4.8
作者：
Metzler, M;Legendre-Guillemin, V;Hayden, MR
通讯作者：
Hayden, MR
影响因子：
4.3
作者：
Guo, Hua-Bei;Johnson, Heather;Pierce, Michael
通讯作者：
Pierce, Michael