Transcripts Expressed during Germination Sensu Stricto Are Associated with Vigor in Soybean Seeds.

Transcripts Expressed during Germination Sensu Stricto Are Associated with Vigor in Soybean Seeds.
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大豆种子萌发过程中表达的转录本与种子活力相关。

DOI:
10.3390/plants11101310
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发表时间:
2022-05-14
期刊:
Plants (Basel, Switzerland)
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通过统一的机械作物收获,在田间迅速和统一地种植作物是粮食安全的基础。为了达到这一目的,应使用具有更大活力的种子。活力是与种子弹性有关的生理品质的组成部分。尽管这一重要性,有很少的知识之间的关联事件在分子水平和种子活力。在这项研究中,我们研究了大豆种子发芽过程中的基因表达和种子活力之间的关系。在萌发过程开始时与生长相关的20个基因的表达水平与活力相关。本文采用不同的试验方法对该菌株的活力进行了评价。然后,我们报告了扩展蛋白样A1,木葡聚糖内切转葡糖基酶/水解酶22,65-kDa微管相关蛋白,木葡聚糖内切转葡糖基酶/水解酶2,N-糖基化酶/DNA裂解酶OGG 1和纤维素合成酶A催化亚基2,这是在萌发过程中表达的基因的鉴定,这与几种常用的大豆种子质量的常规分析的活力测试。这些转录本的鉴定为在分子水平上研究大豆种子活力提供了工具。
The rapid and uniform establishment of crop plants in the field underpins food security through uniform mechanical crop harvesting. In order to achieve this, seeds with greater vigor should be used. Vigor is a component of physiological quality related to seed resilience. Despite this importance, there is little knowledge of the association between events at the molecular level and seed vigor. In this study, we investigated the relationship between gene expression during germination and seed vigor in soybean. The expression level of twenty genes related to growth at the beginning of the germination process was correlated with vigor. In this paper, vigor was evaluated by different tests. Then we reported the identification of the genes Expansin-like A1, Xyloglucan endotransglucosylase/hydrolase 22, 65-kDa microtubule-associated protein, Xyloglucan endotransglucosylase/hydrolase 2, N-glycosylase/DNA lyase OGG1 and Cellulose synthase A catalytic subunit 2, which are expressed during germination, that correlated with several vigor tests commonly used in routine analysis of soybean seed quality. The identification of these transcripts provides tools to study vigor in soybean seeds at the molecular level.
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