Adsorption and Electropolymerization of p-Aminophenol Reduces Reproducibility of Electrochemical Immunoassays.
Adsorption and Electropolymerization of p-Aminophenol Reduces Reproducibility of Electrochemical Immunoassays.
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DOI：
10.3390/molecules27186046
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发表时间：
2022-09-16
期刊：
影响因子：
--
通讯作者：
Cliffel DE
中科院分区：
文献类型：
--
作者：
Buckey G;Owens OE;Gabriel AW;Downing CM;Calhoun MC;Cliffel DE
研究了中性和碱性条件下对氨基苯酚（PAP）在市售碳丝网印刷电极（CSPE）和金丝网印刷电极（GSPE）上的电化学行为，为开发廉价的免疫分析方法奠定了基础。PAP的电化学氧化信号来自其吸附到电极上。在金电极上形成的自组装单分子膜阻止了PAP吸附，但也降低了其氧化电流，证实吸附增加了信号产生。在裸电极上，PAP吸附导致氧化电流变化，这取决于丝网印刷电极的电活性表面积。这种可变性不能通过清洁和重复使用相同的GSPE来补救。将PAP浓度降低至3.8 μM，大大提高了测量的一致性，表明PAP的吸附具有浓度依赖性。在同一电极上的多个PAP氧化引起聚合，限制PAP在连续监测应用中。红外和拉曼光谱允许之间的区别吸附PAP和电聚合PAP的表面上的金晶片。这项研究的结果表明，在使用SPE的免疫测定中使用PAP生产必须进行微调，并且必须在测量之间清洁或处理电极。
This paper investigates the electrochemical behavior of p-aminophenol (PAP) on commercially available carbon screen-printed electrodes (CSPEs) and gold screen-printed electrodes (GSPEs) at neutral and basic pHs for the development of inexpensive immunoassays. The electrochemical oxidative signal from PAP results from its adsorption to the electrode. The formation of self-assembled monolayers on gold electrodes prevented PAP adsorption but also reduced its oxidative current, confirming that adsorption increases signal production. On bare electrodes, PAP adsorption results in oxidative current variability depending on the electroactive surface area of the screen-printed electrode. This variability could not be remedied by cleaning and reusing the same GSPE. Decreasing the PAP concentration to 3.8 μM greatly improved the consistency of the measurements, suggesting that the adsorption of PAP is concentration-dependent. Multiple PAP oxidations on the same electrode caused polymerization, limiting PAP in continuous monitoring applications. Infrared and Raman spectroscopy allow the distinction between adsorbed PAP and electropolymerized PAP on the surface of a gold wafer. The results from this study suggest that the use of PAP production in immunoassays with SPEs must be fine-tuned, and electrodes must be cleaned or disposed of between measurements.
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影响因子：
15
作者：
JOSCHEK, HI;MILLER, SI
通讯作者：
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影响因子：
4.5
作者：
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影响因子：
6.2
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.5
作者：
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