Absence of Host-Specific Genes in Canine and Human Staphylococcus pseudintermedius as Inferred from Comparative Genomics.
Absence of Host-Specific Genes in Canine and Human Staphylococcus pseudintermedius as Inferred from Comparative Genomics.
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DOI：
10.3390/antibiotics10070854
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发表时间：
2021-07-14
期刊：
影响因子：
--
通讯作者：
Duim B
中科院分区：
文献类型：
--
作者：
Wegener A;Broens EM;van der Graaf-van Bloois L;Zomer AL;Visser CE;van Zeijl J;van der Meer C;Kusters JG;Friedrich AW;Kampinga GA;Sips GJ;Smeets L;van Kerckhoven MEJ;Timmerman AJ;Wagenaar JA;Duim B
假中间葡萄球菌是犬类中的一种重要病原体，作为老年人和免疫功能低下人群的机会致病菌，偶尔会引起人类感染。本研究利用全基因组关联研究（GWAS）比较了犬和人假中间假葡萄球菌分离株的基因组亲缘性和耐药基因。犬（n = 25）和人（n = 32）对甲西林敏感的假中间沙门氏菌（MSSP）分离株表现出高度的遗传多样性，克隆复体CC241在人分离株中具有较高的代表性。该克隆复合体携带一个质粒，其中包含一个具有细胞毒性的细菌素、一个CRISPR-cas结构域和一个含有5个抗菌素耐药基因的pre25样移动元件。13例（41%）人分离株和14例（56%）犬分离株预测存在多药耐药（MDR）。CC241代表了预测的人类MDR分离株的54%和预测的犬MDR分离株的21%。虽然以前有人认为存在某些宿主特异性基因，但目前的GWAS分析没有发现任何与人类或犬类分离物显著相关的基因。总之，这是第一次基因组研究表明MSSP在两种宿主中具有遗传多样性，并且多药耐药性在狗和人类相关的假中间假葡萄球菌分离株中很重要。
Staphylococcus pseudintermedius is an important pathogen in dogs that occasionally causes infections in humans as an opportunistic pathogen of elderly and immunocompromised people. This study compared the genomic relatedness and antimicrobial resistance genes using genome-wide association study (GWAS) to examine host association of canine and human S. pseudintermedius isolates. Canine (n = 25) and human (n = 32) methicillin-susceptible S. pseudintermedius (MSSP) isolates showed a high level of genetic diversity with an overrepresentation of clonal complex CC241 in human isolates. This clonal complex was associated with carriage of a plasmid containing a bacteriocin with cytotoxic properties, a CRISPR-cas domain and a pRE25-like mobile element containing five antimicrobial resistance genes. Multi-drug resistance (MDR) was predicted in 13 (41%) of human isolates and 14 (56%) of canine isolates. CC241 represented 54% of predicted MDR isolates from humans and 21% of predicted MDR canine isolates. While it had previously been suggested that certain host-specific genes were present the current GWAS analysis did not identify any genes that were significantly associated with human or canine isolates. In conclusion, this is the first genomic study showing that MSSP is genetically diverse in both hosts and that multidrug resistance is important in dog and human-associated S. pseudintermedius isolates.
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影响因子：
3.7
作者：
Thomsen MC;Ahrenfeldt J;Cisneros JL;Jurtz V;Larsen MV;Hasman H;Aarestrup FM;Lund O
通讯作者：
Lund O
影响因子：
3.2
作者：
Bannoehr, Jeanette;Ben Zakour, Nouri L.;Fitzgerald, J. Ross
通讯作者：
Fitzgerald, J. Ross
影响因子：
2.1
作者：
Lozano, Carmen;Rezusta, Antonio;Torres, Carmen
通讯作者：
Torres, Carmen
影响因子：
5.8
作者：
Page, Andrew J.;Cummins, Carla A.;Parkhill, Julian
通讯作者：
Parkhill, Julian
影响因子：
7
作者：
Parks DH;Imelfort M;Skennerton CT;Hugenholtz P;Tyson GW
通讯作者：
Tyson GW