Autoantibody response to a novel tumor-associated antigen p90/CIP2A in breast cancer immunodiagnosis.
Autoantibody response to a novel tumor-associated antigen p90/CIP2A in breast cancer immunodiagnosis.
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乳腺癌免疫诊断中对新型肿瘤相关抗原 p90/CIP2A 的自身抗体反应
DOI：
10.1007/s13277-013-1350-6
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Zhang, Jian-Ying
中科院分区：
文献类型：
--
作者：
Liu, Xinxin;Chai, Yurong;Li, Jitian;Ren, Pengfei;Liu, Mei;Dai, Liping;Qian, Wei;Li, Wenjie;Zhang, Jian-Ying
迫切需要鉴定对乳腺癌早期免疫诊断具有高敏感性和特异性的相关肿瘤标志物。针对肿瘤相关抗原（TAA）的自身抗体已被证明是相关的肿瘤标志物。本研究的目的是评估肿瘤相关抗原 p90/CIP2A 的自身抗体是否可以用作乳腺癌的诊断标志物。在本研究中，通过酶联免疫吸附测定 (ELISA)、蛋白质印迹法和间接免疫荧光测定对乳腺癌患者和正常人的血清中的 p90/CIP2A 自身抗体反应进行了评估。结果表明，与正常个体的血清（2.3％）相比，p90/CIP2A可以在乳腺癌中诱导相对较高频率的自身抗体反应（19.1％）。乳腺癌组织中p90/CIP2A表达频率显着高于癌旁正常组织（P<0.01）。我们的初步结果表明，针对 p90/CIP2A 的自身抗体可能是早期乳腺癌筛查和诊断的有用血清生物标志物。
There is an urgent need to identify relevant tumor markers showing high sensitivity and specificity for early immunodiagnosis of breast cancer. Autoantibodies directed against tumor-associated antigens (TAAs) have been shown to be relevant tumor markers. The purpose of this study was to evaluate whether autoantibodies to a tumor-associated antigen p90/CIP2A can be used as diagnostic markers in breast cancer. In this study, autoantibody responses to p90/CIP2A were evaluated by enzyme-linked immunosorbent assay (ELISA), western blotting, and indirect immunofluorescence assay in sera from patients with breast cancer and normal human individuals. The results have demonstrated that p90/CIP2A can induce a relatively higher frequency of autoantibody response in breast cancer (19.1 %) compared to the sera of normal individuals (2.3 %). The frequency of p90/CIP2A expression in breast cancer tissues was significantly higher than that in adjacent normal tissues (P <0.01). Our preliminary results suggest that autoantibodies against p90/CIP2A may be a useful serum biomarker for early stage breast cancer screening and diagnosis.
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