The role of Siglec-1 and SR-BI interaction in the phagocytosis of oxidized low density lipoprotein by macrophages.
The role of Siglec-1 and SR-BI interaction in the phagocytosis of oxidized low density lipoprotein by macrophages.
复制标题
Siglec-1 和 SR-BI 相互作用在巨噬细胞吞噬氧化低密度脂蛋白中的作用
DOI：
10.1371/journal.pone.0058831
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhong RQ
中科院分区：
文献类型：
--
作者：
Xiong YS;Yu J;Li C;Zhu L;Wu LJ;Zhong RQ
巨噬细胞通过oxLDL摄取在动脉粥样硬化中发挥促动脉粥样硬化作用。作为I型凝集素的粘附分子，Siglec-1在动脉粥样硬化患者的循环单核细胞和斑块巨噬细胞上高度表达，但Siglec-1的确切作用尚未阐明。在这项研究中，oxLDL用于刺激骨髓源性巨噬细胞上的Siglec-1和一些oxLDL受体（SR-BI、CD 64、CD 32 B、LOX-1和TLR-4）表达，而小干扰RNA用于下调Siglec-1。同时，进行基于ELISA的Siglec-1-oxLDL相互作用的测定，并使用免疫共沉淀（co-IP）和激光扫描共聚焦显微镜（LSCM）来确定Siglec-1在巨噬细胞摄取oxLDL中的作用。我们发现oxLDL可以剂量依赖性方式上调各种潜在oxLDL受体的表达，包括Siglec-1。下调Siglec-1表达可降低Oil red O染色显示的oxLDL摄取。LSCM显示Siglec-1和CD 64/SR-BI可以共定位于oxLDL刺激的巨噬细胞表面，而co-IP显示Siglec-1和SR-BI可以相互免疫沉淀。然而，在体外蛋白质相互作用系统中没有发现Siglec-1与oxLDL之间的直接相互作用。因此，Siglec-1可以在巨噬细胞吞噬oxLDL中与SR-BI相互作用，而不是作为oxLDL的独立受体。
Macrophages play a proatherosclerotic role in atherosclerosis via oxLDL uptake. As an adhesion molecular of I-type lectins, Siglec-1 is highly expressed on circulating monocytes and plaque macrophages of atherosclerotic patients, but the exact role of Siglec-1 has not been elucidated. In this study, oxLDL was used to stimulate Siglec-1 and some oxLDL receptors (SR-BI, CD64, CD32B, LOX-1 and TLR-4) expression on bone marrow-derived macrophages, whereas small interfering RNA was used to down-regulate Siglec-1. Meanwhile, an ELISA-based assay for Siglec-1-oxLDL interaction was performed, and co-immunoprecipitation (co-IP) and laser scanning confocal microscopy (LSCM) were used to determine the role of Siglec-1 in oxLDL uptake by macrophages. We found that oxLDL could up-regulate the expression of various potential oxLDL receptors, including Siglec-1, in a dose-dependent manner. Moreover, down-regulation of Siglec-1 could attenuate oxLDL uptake by Oil red O staining. LSCM revealed that Siglec-1 and CD64/SR-BI may colocalize on oxLDL-stimulated macrophage surface, whereas co-IP showed that Siglec-1 and SR-BI can be immunoprecipitated by each other. However, no direct interaction between Siglec-1 and oxLDL was found in the in vitro protein interaction system. Thus, Siglec-1 can interact with SR-BI in the phagocytosis of oxLDL by macrophages, rather than act as an independent receptor for oxLDL.
登录
查看更多内容
影响因子：
2.7
作者：
Van Gorp, Hanne;Van Breedam, Wander;Nauwynck, Hans J.
通讯作者：
Nauwynck, Hans J.
影响因子：
9.3
作者：
Fujita, Yoshiko;Kakino, Akemi;Sawamura, Tatsuya
通讯作者：
Sawamura, Tatsuya
影响因子：
3.7
作者：
Rempel H;Calosing C;Sun B;Pulliam L
通讯作者：
Pulliam L
影响因子：
7.7
作者：
Janke, J;Engeli, S;Sharma, AM
通讯作者：
Sharma, AM
DOI：
10.1073/pnas.0609671104
发表时间：
2006-12-26
影响因子：
11.1
作者：
Seimon, Tracie A.;Obstfeld, Amrom;Tabas, Ira
通讯作者：
Tabas, Ira