Analysis of the cell death-inducing ability of the ethylene response factors in group VIII of the AP2/ERF family.
Analysis of the cell death-inducing ability of the ethylene response factors in group VIII of the AP2/ERF family.
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AP2/ERF家族第VIII组乙烯反应因子诱导细胞死亡的能力分析。
DOI：
10.1016/j.plantsci.2013.04.003
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Y. Matsushita
中科院分区：
文献类型：
--
作者：
T. Ogata;Y. Kida;M. Tochigi;Y. Matsushita
乙烯反应因子（ERF）家族是植物特异性转录因子中最大的家族之一。我们之前已经表明，烟草转录抑制因子NtERF3基因的过表达，在c端区域含有erf相关的两亲性抑制（EAR）基序，诱导过敏反应（HR）样细胞死亡。许多含有EAR基序的ERF，包括NtERF3，聚集在ERF家族的第八组。在本研究中，我们旨在揭示viiierf组的细胞诱导死亡能力及其与HR的相关性。结果表明，拟南芥、水稻和烟草中许多含有EAR基序的erf在烟叶中具有诱导细胞死亡的能力。SevenAtERFsin亚组VIII-b不诱导细胞死亡；而水稻和烟草的某些erfsin亚群VIII-b则表现出诱导细胞死亡的能力。通过对诱导HR的烟草中viiierfs组的表达分析，表明其诱导细胞死亡的能力与诱导HR并不一定相关。此外，我们还发现含有EAR基序的terfsin II-a亚组也具有诱导细胞死亡的能力。本文还讨论了EAR基序序列变化对诱导细胞死亡能力的影响。
The ethylene response factor (ERF) family is one of the largest families of plant-specific transcription factors. We have shown previously that the overexpression of the gene for NtERF3, a tobacco transcriptional repressor containing the ERF-associated amphiphilic repression (EAR) motif in the C-terminal region, induces hypersensitive reaction (HR)-like cell death. Many EAR motif-containing ERFs, including NtERF3, are clustered in group VIII of the ERF family. In this study, we aimed at revealing the cell death-inducing ability of group VIIIERFsand the correlation betweenERFsand HR. The results showed that many of the EAR motif-containing ERFs classified into subgroup VIII-a ofArabidopsis, rice, and tobacco had cell death-inducing ability in tobacco leaves. SevenAtERFsin subgroup VIII-b did not induce cell death; however, someERFsin subgroup VIII-b of rice and tobacco showed cell death-inducing ability. An expression analysis of group VIIIERFsin HR-inducing tobacco suggested that the cell death-inducing ability ofNtERFswas not necessarily associated with induction of HR. In addition, it was revealed that the EAR motif-containingAtERFsin subgroup II-a also showed cell death-inducing ability. The influence of sequence variation in the EAR motif on the ability to induce cell death is also discussed.
影响因子：
6.9
作者：
Mur, Luis A. J.;Kenton, Paul;Prats, Elena
通讯作者：
Prats, Elena