Improved curdlan fermentation process based on optimization of dissolved oxygen combined with pH control and metabolic characterization of Agrobacterium sp. ATCC 31749
Improved curdlan fermentation process based on optimization of dissolved oxygen combined with pH control and metabolic characterization of Agrobacterium sp. ATCC 31749
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基于溶解氧优化结合pH控制和农杆菌代谢特性改进凝胶多糖发酵工艺。
DOI：
10.1007/s00253-011-3448-3
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
张洪涛
中科院分区：
文献类型：
--
作者：
郑志永;吴剑荣;詹晓北;张洪涛
规模缩小的培养物中的一个重要问题是存在溶解氧（DO）梯度和pH控制，很少研究代谢表征和产生可德兰的农杆菌ATCC 31749。在分批发酵期间，通过用PID系统操纵搅拌来维持恒定的DO，在5%和75%之间。发酵，代谢和动力学特性的研究进行了规模缩小的系统。溶解氧浓度对凝胶多糖产量、胞内核苷酸水平和葡萄糖转化为凝胶多糖的效率有显著影响。产凝胶多糖的最佳溶解氧浓度为45 - 60%。与15%DO相比，平均可得然胶产量、可得然胶生产率和葡萄糖转化为可得然胶的效率分别提高了80%、66%和32%。没有检测到所得凝胶多糖的凝胶强度的明显差异。比较凝胶多糖的生物合成和细胞内核苷酸水平，发现凝胶多糖的合成与胞内UTP、ADP、AMP、NAD（+）、NADH和UDP-葡萄糖的水平呈正相关。45%DO和60%DO下的凝胶多糖产率在20-50 h内存在差异。然而，60小时后，两种条件下的可得然胶生产率相似。在此基础上，开发了一种简单、重现性好的可得然胶生产的两段式溶氧控制工艺。可得然胶产量达到42.8g/l，比单一搅拌速度控制工艺提高了30%。
A significant problem in scale-down cultures, rarely studied for metabolic characterization and curdlan-producing Agrobacterium sp. ATCC 31749, is the presence of dissolved oxygen (DO) gradients combined with pH control. Constant DO, between 5% and 75%, was maintained during batch fermentations by manipulating the agitation with PID system. Fermentation, metabolic and kinetic characterization studies were conducted in a scale-down system. The curdlan yield, intracellular nucleotide levels and glucose conversion efficiency into curdlan were significantly affected by DO concentrations. The optimum DO concentrations for curdlan production were 45-60%. The average curdlan yield, curdlan productivity and glucose conversion efficiency into curdlan were enhanced by 80%, 66% and 32%, respectively, compared to that at 15% DO. No apparent difference in the gel strength of the resulting curdlan was detected. The comparison of curdlan biosynthesis and cellular nucleotide levels showed that curdlan production had positive relationship with intracellular levels of UTP, ADP, AMP, NAD(+), NADH and UDP-glucose. The curdlan productivity under 45% DO and 60% DO was different during 20-50 h. However, after 60 h curdlan productivity of both conditions was similar. On that basis, a simple and reproducible two-stage DO control process for curdlan production was developed. Curdlan production yield reached 42.8 g/l, an increase of 30% compared to that of the single agitation speed control process.
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影响因子：
5
作者：
Wu, Jian-Rong;Jiang Yun;Zhang, Hong-Tao;Lin, Chi Chung;Yu, Xiao-Bing;Zheng, Zhi-Yong;Zhan, Xiaobei
通讯作者：
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影响因子：
2.7
作者：
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通讯作者：
M. Moscovici;C. Ionescu;C. Oniscu;Ortansa Fotea;Paula Protopopescu;Lucian Dorin Hanganu
影响因子：
4.4
作者：
Kumar, Himanshu;Kumagai, Yutaro;Kawai, Taro
通讯作者：
Kawai, Taro
影响因子：
4.1
作者：
Jin, Li-Hua;Um, Hyun-Ju;Lee, Jung-Heon
通讯作者：
Lee, Jung-Heon
影响因子：
3.4
作者：
García-Ochoa, F;Castro, EG;Santos, VE
通讯作者：
Santos, VE