Cyclic AMP-Protein Kinase A and Snf1 Signaling Mechanisms Underlie the Superior Potency of Sucrose for Induction of Filamentation in Saccharomyces cerevisiae
Cyclic AMP-Protein Kinase A and Snf1 Signaling Mechanisms Underlie the Superior Potency of Sucrose for Induction of Filamentation in Saccharomyces cerevisiae
复制标题
环 AMP 蛋白激酶 A 和 Snf1 信号传导机制是蔗糖诱导酿酒酵母丝状形成的卓越功效的基础
作者：
S. Van de Velde;J. Thevelein
摘要在特定的环境条件下，酿酒酵母（Saccharomycescerevisiae）可以发生形态转变，形成假菌丝生长模式。假菌丝的分化通常是在葡萄糖存在下通过氮限制诱导的。已知它受几种信号通路控制，包括促分裂原活化蛋白激酶、环AMP-蛋白激酶A（cAMP-PKA）和Snf 1激酶通路。我们表明，α-葡萄糖苷糖麦芽糖和麦芽三糖，特别是蔗糖，是更有效的诱导剂比葡萄糖。蔗糖甚至在富氮培养基中和在铵限制培养基上的mep 2 Δ/mep 2 Δ铵通透酶突变体中诱导发酵。我们证明，葡萄糖也抑制通过一个途径平行的cAMP-PKA途径的抑制作用。HXK 2的缺失将葡萄糖上的假菌丝生长模式转移到蔗糖上，而SNF 4的缺失废除了两种糖上的抑制，表明葡萄糖抑制的负面作用和Snf 1活性在控制抑制中的积极作用。在所有菌株和所有培养基中，蔗糖/葡萄糖敏感G-蛋白偶联受体Gpr 1的缺失大大减少了蔗糖诱导的表达，而它对麦芽糖和麦芽三糖的诱导没有影响。α-葡糖苷糖诱导絮凝作用的能力反映在细胞表面絮凝蛋白基因FLO 11的表达增加中。此外，蔗糖是唯一能够以Gpr 1依赖性方式快速诱导FLO 11表达的α-葡萄糖苷糖，反映了Gpr 1对这种糖的敏感性及其参与快速蔗糖信号传导。我们的研究确定了蔗糖作为最有效的营养诱导剂的假菌丝生长，并表明葡萄糖失活的Snf 1激酶信号是负责葡萄糖的效力较低。
ABSTRACT Under specific environmental conditions, the yeast Saccharomyces cerevisiae can undergo a morphological switch to a pseudohyphal growth pattern. Pseudohyphal differentiation is generally studied upon induction by nitrogen limitation in the presence of glucose. It is known to be controlled by several signaling pathways, including mitogen-activated protein kinase, cyclic AMP-protein kinase A (cAMP-PKA), and Snf1 kinase pathways. We show that the alpha-glucoside sugars maltose and maltotriose, and especially sucrose, are more potent inducers of filamentation than glucose. Sucrose even induces filamentation in nitrogen-rich media and in the mep2Δ/mep2Δ ammonium permease mutant on ammonium-limiting medium. We demonstrate that glucose also inhibits filamentation by means of a pathway parallel to the cAMP-PKA pathway. Deletion of HXK2 shifted the pseudohyphal growth pattern on glucose to that of sucrose, while deletion of SNF4 abrogated filamentation on both sugars, indicating a negative role of glucose repression and a positive role for Snf1 activity in the control of filamentation. In all strains and in all media, sucrose induction of filamentation is greatly diminished by deletion of the sucrose/glucose-sensing G-protein-coupled receptor Gpr1, whereas it has no effect on induction by maltose and maltotriose. The competence of alpha-glucoside sugars to induce filamentation is reflected in the increased expression of the cell surface flocculin gene FLO11. In addition, sucrose is the only alpha-glucoside sugar capable of rapidly inducing FLO11 expression in a Gpr1-dependent manner, reflecting the sensitivity of Gpr1 for this sugar and its involvement in rapid sucrose signaling. Our study identifies sucrose as the most potent nutrient inducer of pseudohyphal growth and shows that glucose inactivation of Snf1 kinase signaling is responsible for the lower potency of glucose.
影响因子：
10.5
作者：
Chen, H;Fink, GR
通讯作者：
Fink, GR
影响因子：
10.5
作者：
Jiang, R;Carlson, M
通讯作者：
Carlson, M