Total synthesis of a functional designer eukaryotic chromosome.
Total synthesis of a functional designer eukaryotic chromosome.
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DOI：
10.1126/science.1249252
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发表时间：
2014-04-04
期刊：
影响因子：
--
通讯作者：
Chandrasegaran S
中科院分区：
文献类型：
--
作者：
Annaluru N;Muller H;Mitchell LA;Ramalingam S;Stracquadanio G;Richardson SM;Dymond JS;Kuang Z;Scheifele LZ;Cooper EM;Cai Y;Zeller K;Agmon N;Han JS;Hadjithomas M;Tullman J;Caravelli K;Cirelli K;Guo Z;London V;Yeluru A;Murugan S;Kandavelou K;Agier N;Fischer G;Yang K;Martin JA;Bilgel M;Bohutski P;Boulier KM;Capaldo BJ;Chang J;Charoen K;Choi WJ;Deng P;DiCarlo JE;Doong J;Dunn J;Feinberg JI;Fernandez C;Floria CE;Gladowski D;Hadidi P;Ishizuka I;Jabbari J;Lau CY;Lee PA;Li S;Lin D;Linder ME;Ling J;Liu J;Liu J;London M;Ma H;Mao J;McDade JE;McMillan A;Moore AM;Oh WC;Ouyang Y;Patel R;Paul M;Paulsen LC;Qiu J;Rhee A;Rubashkin MG;Soh IY;Sotuyo NE;Srinivas V;Suarez A;Wong A;Wong R;Xie WR;Xu Y;Yu AT;Koszul R;Bader JS;Boeke JD;Chandrasegaran S
DNA合成技术的快速进步使我们可以在这里设计病毒，生化途径和组装细菌基因组。对Syliii的更改包括标签/TAA停止型替代品，删除亚电位区域，方法TRNA，转座子和静音位点以及loxpsym位点的插入使基因组在酿酒酵母中起作用。 Syliii的Matα等位基因。用于设计师真核基因组生物学。
Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871 bp designer eukaryotic chromosome, synIII, which is based on the 316,617 bp native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, tRNAs, transposons and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling. SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in “a mater” derivatives resulting from loss of the MATα allele on synIII. The total synthesis of synIII represents the first complete design and synthesis of a eukaryotic chromosome, establishing S. cerevisiae as the basis for designer eukaryotic genome biology.
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影响因子：
64.8
作者：
Lengronne, A;Katou, Y;Mori, S;Yokobayashi, S;Kelly, GP;Itoh, T;Watanabe, Y;Shirahige, K;Uhlmann, F
通讯作者：
Uhlmann, F
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
DOI：
10.1007/978-1-61779-564-0_7
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Annaluru, Narayana;Muller, Heloise;Chandrasegaran, Srinivasan
通讯作者：
Chandrasegaran, Srinivasan
影响因子：
10.5
作者：
D'Ambrosio, Claudio;Schmidt, Christine Katrin;Uhlmann, Frank
通讯作者：
Uhlmann, Frank
影响因子：
64.8
作者：
Wang HH;Isaacs FJ;Carr PA;Sun ZZ;Xu G;Forest CR;Church GM
通讯作者：
Church GM