Cohesin relocation from sites of chromosomal loading to places of convergent transcription.
Cohesin relocation from sites of chromosomal loading to places of convergent transcription.
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DOI：
10.1038/nature02742
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发表时间：
2004-07-29
期刊：
影响因子：
64.8
通讯作者：
Uhlmann, F
中科院分区：
文献类型：
--
作者：
Lengronne, A;Katou, Y;Mori, S;Yokobayashi, S;Kelly, GP;Itoh, T;Watanabe, Y;Shirahige, K;Uhlmann, F
姐妹染色单体是真核DNA复制的产物，通过染色体粘着蛋白复合物的合成后，将其合成。通过环绕，但是在这里，粘蛋白结合到体内的染色体仍然是投票的。酵母菌染色体III - VI几乎在融合方向上转录的基因之间的粘着蛋白。 SCC4粘蛋白载荷复合物，从那里滑到其更永久的位置。建立的转录变化导致粘蛋白的重新定位。它是真核染色体的共同特征。
Sister chromatids, the products of eukaryotic DNA replication, are held together after their synthesis by the chromosomal cohesin complex. This allows the spindle in mitosis to recognise pairs of replication products for segregation into opposite direction. Cohesin forms large protein rings that may bind DNA strands by encircling, but the characterisation of cohesin binding to chromosomes in vivo has remained vague. Here, we present high resolution analysis of cohesin association along budding yeast chromosomes III – VI. Cohesin localises almost exclusively between genes transcribed in converging direction. We find that not the underlying sequence, but active transcription positions cohesin at these sites. Cohesin is initially loaded onto chromosomes at separate places, marked by the Scc2/Scc4 cohesin loading complex, from where it appears to slide to its more permanent locations. But even after sister chromatid cohesion is established changes in transcription lead to repositioning of cohesin. Thus a key architectural feature of mitotic chromosomes, the sites of cohesin binding and therefore most likely sister chromatid cohesion, display surprising flexibility. Cohesin localisation to places of convergent transcription is conserved in fission yeast, suggesting that it is a common feature of eukaryotic chromosomes.
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影响因子：
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Kleckner, N
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Laloraya, S;Guacci, V;Koshland, D
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