Characterization of TsMRP-L28, a mitochondrial ribosomal protein L28 from the parasitic nematode Trichinella spiralis.
Characterization of TsMRP-L28, a mitochondrial ribosomal protein L28 from the parasitic nematode Trichinella spiralis.
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TsMRP-L28（一种来自寄生线虫旋毛虫的线粒体核糖体蛋白 L28）的表征。
DOI：
10.1016/j.gene.2012.10.010
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发表时间：
2013-01
期刊：
影响因子：
3.5
通讯作者：
Tian, Guimei
中科院分区：
文献类型：
--
作者：
杨玉荣;Jian, Wei;Qin, Weiwen;Tian, Guimei
从旋毛虫（Trichinellaspiralis）中分离并克隆了一个编码线粒体核糖体蛋白（MRP）-L28的cDNA。预测的TsMRP-L28蛋白由276个氨基酸组成。系统进化分析表明TsMRP-L28与秀丽隐杆线虫线粒体核糖体蛋白L28有很近的亲缘关系。TsMRP-L28转录本在新生幼虫、肌幼虫和雄性和雌性蠕虫中表达。Western blot结果显示TsMRP-L28在肌幼虫和蠕虫中均有表达。免疫定位结果显示，TsMRP-L28在新生幼虫、肌肉幼虫和蠕虫中广泛分布，在新生幼虫和不同发育阶段的胚胎细胞质中富集，且在蛋白合成活性较高的细胞中富集。这些数据表明TsMRP-L28是T.螺旋。
A cDNA encoding mitochondrial ribosomal protein (MRP)-L28 was isolated and cloned from Trichinella spiralis, an economically important parasitic nematode. The predicted TsMRP-L28 protein consists of 276 amino acids. Phylogenetic analysis revealed that TsMRP-L28 was closely related to Caenorhabditis elegans mitochondrial ribosomal protein L28. The TsMRP-L28 transcript was expressed in newborn larvae, muscle larvae and male and female adult worms. Western blot showed that TsMRP-L28 was expressed in muscle larvae and adult worms. Immuno-localization revealed that TsMRP-L28 was ubiquitously distributed in newborn larvae, muscle larvae and adult worms, and that TsMRP-L28 was enriched in cells with higher protein synthesis activity, such as in newborn larvae and the cytoplasm of different developmental stages of embryos. These data suggest that TsMRP-L28 is required for the early development of T. spiralis.
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影响因子：
2
作者：
R. Dmochowski
通讯作者：
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DOI：
10.1016/j.respe.2020.09.004
发表时间：
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期刊：
Revue D'Epidemiologie et De Sante Publique
影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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