Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum.
Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum.
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DOI：
10.1186/1754-6834-4-25
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发表时间：
2011-08-24
影响因子：
6.3
通讯作者：
Zhao ZK
中科院分区：
文献类型：
--
作者：
Hu C;Wu S;Wang Q;Jin G;Shen H;Zhao ZK
木质纤维素水解物的生化转化仍然具有挑战性，这主要是因为大多数微生物过程在同时存在己糖和戊糖的情况下效率显著降低。因此，鉴定能够同时高效利用葡萄糖和木糖的微生物是改进这一过程的关键。在本研究中，我们发现含油性酵母菌株Trichosporon cutaneum as 2.571同时吸收葡萄糖和木糖，并在3升搅拌槽生物反应器中以2：1的葡萄糖/木糖混合物培养，细胞内脂肪积累高达59wt%，脂肪系数高达0.17g/g糖。此外，没有观察到典型的营养生长行为模式；微生物细胞量在整个培养过程中增加，没有任何停滞期。在具有不同初始葡萄糖：木糖比例的摇瓶培养中，葡萄糖和木糖以大致与各自在培养基中的浓度成正比的速度同时消耗，导致两种糖的完全利用。玉米秸秆水解液在发酵过程中同时利用葡萄糖和木糖，其脂肪含量和系数分别为39.2%和0.15 g/g糖。所得油脂的脂肪酸组成与传统植物油相似，具有作为生物柴油生产原料的潜力。本研究实现了葡萄糖和木糖同时消耗的高效脂肪生产。这一过程提供了一个令人兴奋的机会，将木质纤维材料转化为生物燃料分子，并应鼓励进一步研究，以阐明这一独特的糖同化机制。
Biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence of both hexoses and pentoses. Thus, identification of microorganisms capable of efficient and simultaneous utilization of both glucose and xylose is pivotal to improving this process. In this study, we found that the oleaginous yeast strain Trichosporon cutaneum AS 2.571 assimilated glucose and xylose simultaneously, and accumulated intracellular lipid up to 59 wt% with a lipid coefficient up to 0.17 g/g sugar, upon cultivation on a 2:1 glucose/xylose mixture in a 3-liter stirred-tank bioreactor. In addition, no classic pattern of diauxic growth behavior was seen; the microbial cell mass increased during the whole culture process without any lag periods. In shake-flask cultures with different initial glucose:xylose ratios, glucose and xylose were consumed simultaneously at rates roughly proportional to their individual concentrations in the medium, leading to complete utilization of both sugars at the same time. Simultaneous utilization of glucose and xylose was also seen during fermentation of corn-stover hydrolysate with a lipid content and coefficient of 39.2% and 0.15 g/g sugar, respectively. The lipid produced had a fatty-acid compositional profile similar to those of conventional vegetable oil, indicating that it could have potential as a raw material for biodiesel production. Efficient lipid production with simultaneous consumption of glucose and xylose was achieved in this study. This process provides an exciting opportunity to transform lignocellulosic materials into biofuel molecules, and should also encourage further study to elucidate this unique sugar-assimilation mechanism.
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影响因子：
4.4
作者：
HSIAO, HY;CHIANG, LC;TSAO, GT
通讯作者：
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影响因子：
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