High performance micro-flow cytometer based on optical fibres.
High performance micro-flow cytometer based on optical fibres.
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DOI：
10.1038/s41598-017-05843-7
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发表时间：
2017-07-17
影响因子：
4.6
通讯作者：
Russom A
中科院分区：
文献类型：
--
作者：
Etcheverry S;Faridi A;Ramachandraiah H;Kumar T;Margulis W;Laurell F;Russom A
流式细胞术目前是医学实验室和生物医学研究中细胞分析的金标准。在护理点诊断的需要的推动下，已经做出了显著的努力来简化和降低流式细胞仪的成本。然而，尽管最近取得了进展，目前的微系统仍然不太通用，比大规模的同行慢得多。在这项工作中，提出了一种全硅纤维微流式细胞仪，测量颗粒和细胞的荧光和散射。它整合了圆形毛细管中的细胞运输和光纤的光传输。单流细胞聚焦由弹性惯性微流体执行，以保证准确和灵敏的检测。该技术的能力扩展到高流速（高达800 µl/min），使吞吐量达到2500个颗粒/s。这里描述的强大的，便携式和低成本的系统可以是一个点的护理流式细胞仪与商业系统的性能相当的基础。
Flow cytometry is currently the gold standard for analysis of cells in the medical laboratory and biomedical research. Fuelled by the need of point-of-care diagnosis, a significant effort has been made to miniaturize and reduce cost of flow cytometers. However, despite recent advances, current microsystems remain less versatile and much slower than their large-scale counterparts. In this work, an all-silica fibre microflow cytometer is presented that measures fluorescence and scattering from particles and cells. It integrates cell transport in circular capillaries and light delivery by optical fibres. Single-stream cell focusing is performed by Elasto-inertial microfluidics to guarantee accurate and sensitive detection. The capability of this technique is extended to high flow rates (up to 800 µl/min), enabling a throughput of 2500 particles/s. The robust, portable and low-cost system described here could be the basis for a point-of-care flow cytometer with a performance comparable to commercial systems.
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