H(2)S-mediated balance regulation of stomatal and non-stomatal factors responding to drought stress in Chinese cabbage.
H(2)S-mediated balance regulation of stomatal and non-stomatal factors responding to drought stress in Chinese cabbage.
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DOI：
10.1093/hr/uhac284
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发表时间：
2023-03
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
作者：
越来越多的证据表明，硫化氢（H2S）作为一种新型气体递质，可以通过诱导气孔关闭来增强植物的抗旱性，同时提高光合作用效率，但人们对这种矛盾现象背后的机制知之甚少。本研究探讨了大白菜中 H2S 响应气孔和非气孔因素干旱胁迫的调节机制。结果表明，外源H2S可以增加光合色素的积累，减轻干旱胁迫造成的损害。它还在干旱胁迫下调节转录水平的表达和核酮糖1,5-二磷酸羧化酶/加氧酶（BrRuBisCO）的活性。 BrRuBisCO的大亚基被发现被S-硫化修饰，这可能是其酶活性增加的原因。在干旱胁迫下，通过非侵入性微测试技术检测保卫细胞中的 Cl−、K+ 和 H+ 通量。结果表明，H2S 信号传导诱导跨膜 Cl− 和 H+ 流出并抑制 K+ 流入，并且 Cl− 通道是 H2S 调节气孔运动的主要响应者。总之，H2S信号不仅激活位于保卫细胞膜上的离子通道蛋白以诱导气孔关闭，而且还调节非气孔因子RuBisCO的转录表达和活性，从而提高叶片的光合效率。因此，由于干旱胁迫，H2S 信号对气孔因子和非气孔因子的调节之间存在有益的平衡，需要更好地理解这一点，以便将其实际应用到提高作物产量。
Increased evidence has shown that hydrogen sulfide (H2S), a novel gasotransmitter, could enhance drought resistance in plants by inducing stomatal closure, with concurrent enhancement of photosynthetic efficiency, but little is known about the mechanism behind this contradictory phenomenon. This study examined the regulating mechanism of H2S in response to drought stress from stomatal and non-stomatal factors in Chinese cabbage. The results showed that exogenous H2S could increase the accumulation of photosynthetic pigments and alleviate the damage caused by drought stress. It also regulated the expression in transcriptional level and the activity of ribulose 1,5-bisphosphate carboxylase/oxygenase (BrRuBisCO) under drought stress. The large subunit of BrRuBisCO was found to be modified by S-sulfhydration, which might be the reason for its increased enzyme activity. The fluxes of Cl−, K+, and H+ in the guard cells were detected by non-invasive micro-test techniques while under drought stress. The results indicated that H2S signaling induced a transmembrane Cl− and H+ efflux and inhibited K+ influx, and the Cl− channel was the main responders for H2S-regulated stomatal movement. In conclusion, H2S signal not only activated the ion channel proteins located in the guard cell membrane to induce stomatal closure, but also regulated the transcriptional expression and the activity of RuBisCO, a non-stomatal factor to enhance the photosynthetic efficiency of leaves. There is therefore a beneficial balance between the regulation of H2S signaling on stomatal factors and non-stomatal factors due to drought stress, which needs to be better understood to apply it practically to increase crop yields.
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