Priming adult stem cells by hypoxic pretreatments for applications in regenerative medicine.
Priming adult stem cells by hypoxic pretreatments for applications in regenerative medicine.
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DOI：
10.1186/1423-0127-20-63
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发表时间：
2013-08-29
影响因子：
11
通讯作者：
Guarnieri C
中科院分区：
文献类型：
--
作者：
Muscari C;Giordano E;Bonafè F;Govoni M;Pasini A;Guarnieri C
在干细胞移植前提高其生物学性能可以提高再生医学的效率。几种非损伤性细胞缺氧的离体方案已被证明可显著增加干细胞的存活、增殖和移植后分化潜力。根据严重缺血器官中缺血预处理的常规操作所提供的非凡保护，通过短暂间歇性缺氧或缺氧和再氧，已经获得了针对随后的、否则致命的缺血应激的预处理培养干细胞的最佳结果。这些协议的低氧预处理可以很容易地在实验室中重现，然而，更合适的药理学干预诱导干细胞反应类似于那些在缺氧中激活被认为是最有前途的解决方案，为未来的细胞治疗中的应用。在这里，我们想提供一个最新的审查的分子机制翻译缺氧到有益的事件再生医学。为此，将讨论主要由缺氧诱导因子激活的表观遗传修饰、microRNA和氧化应激的参与。还将考虑干细胞对健康和肿瘤组织中天然缺氧微环境（生态位）的适应。
The efficiency of regenerative medicine can be ameliorated by improving the biological performances of stem cells before their transplantation. Several ex-vivo protocols of non-damaging cell hypoxia have been demonstrated to significantly increase survival, proliferation and post-engraftment differentiation potential of stem cells. The best results for priming cultured stem cells against a following, otherwise lethal, ischemic stress have been obtained with brief intermittent episodes of hypoxia, or anoxia, and reoxygenation in accordance with the extraordinary protection afforded by the conventional maneuver of ischemic preconditioning in severely ischemic organs. These protocols of hypoxic preconditioning can be rather easily reproduced in a laboratory; however, more suitable pharmacological interventions inducing stem cell responses similar to those activated in hypoxia are considered among the most promising solutions for future applications in cell therapy. Here we want to offer an up-to-date review of the molecular mechanisms translating hypoxia into beneficial events for regenerative medicine. To this aim the involvement of epigenetic modifications, microRNAs, and oxidative stress, mainly activated by hypoxia inducible factors, will be discussed. Stem cell adaptation to their natural hypoxic microenvironments (niche) in healthy and neoplastic tissues will be also considered.
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影响因子：
7.7
作者：
Emamaullee, Juliet A.;Shapiro, A. M. James
通讯作者：
Shapiro, A. M. James
影响因子：
11.2
作者：
Chen, Haobin;Yan, Yan;Costa, Max
通讯作者：
Costa, Max
影响因子：
11.5
作者：
Daneau, Geraldine;Boidot, Romain;Feron, Olivier
通讯作者：
Feron, Olivier
DOI：
10.1016/s0034-5687(01)00306-1
发表时间：
2001-11-15
期刊：
RESPIRATION PHYSIOLOGY
影响因子：
--
作者：
Erecinska, M;Silver, IA
通讯作者：
Silver, IA
缺血性脑损伤的干细胞：批判性综述。
DOI：
10.1002/cne.22038
发表时间：
2009-07-01
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Burns TC;Verfaillie CM;Low WC
通讯作者：
Low WC