ARTEMISININ BIOSYNTHESIS PROMOTING KINASE 1 positively regulates artemisinin biosynthesis through phosphorylating AabZIP1.
ARTEMISININ BIOSYNTHESIS PROMOTING KINASE 1 positively regulates artemisinin biosynthesis through phosphorylating AabZIP1.
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青蒿素生物合成促进激酶 1 通过磷酸化 AabZIP1 正向调节青蒿素生物合成
DOI：
10.1093/jxb/erx444
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发表时间：
2018-02-23
影响因子：
6.9
通讯作者：
Liao Z
中科院分区：
文献类型：
--
作者：
Zhang F;Xiang L;Yu Q;Zhang H;Zhang T;Zeng J;Geng C;Li L;Fu X;Shen Q;Yang C;Lan X;Chen M;Tang K;Liao Z
ABA响应性SnRK2型激酶AaAPK1通过磷酸化AabZIP1参与青蒿素生物合成的正调控。植物黄花蒿产生抗疟疾化合物青蒿素。虽然青蒿素生物合成的转录调控已被广泛研究，但其翻译后调控机制，特别是蛋白磷酸化的机制仍不清楚。在这里，我们报告说，ABA响应激酶（AaAPK1），SnRK2家族的成员，参与调节青蒿素的生物合成。AaAPK1与AabZIP1的物理相互作用通过多种测定法证实，包括酵母双杂交、双分子荧光互补和下拉。AaAPK 1主要在花蕾和叶片中表达，阿坝、干旱和NaCl处理均可诱导其表达。Phos标记迁移率变动分析表明，AaAPK1磷酸化本身和AabZIP1。因此，磷酸化的AaAPK 1显著增强了AabZIP 1对青蒿素生物合成基因的反式激活活性。用Ala37取代AabZIP1的Ser37显著抑制其磷酸化，从而抑制AabZIP1的反式激活活性。AaAPK1的持续过表达显著增加了青蒿素的产量，以及青蒿素生物合成基因的表达水平。我们的研究打开了一个窗口，在翻译后水平上的青蒿素生物合成的调控网络。重要的是，我们第一次为激酶基因AaAPK1为什么是青蒿素生物合成代谢工程的关键候选人提供了证据。
An ABA-responsive SnRK2-type kinase, AaAPK1, is involved in positively regulating artemisinin biosynthesis in Artemisia annua through phosphorylation of AabZIP1. The plant Artemisia annua produces the anti-malarial compound artemisinin. Although the transcriptional regulation of artemisinin biosynthesis has been extensively studied, its post-translational regulatory mechanisms, especially that of protein phosphorylation, remain unknown. Here, we report that an ABA-responsive kinase (AaAPK1), a member of the SnRK2 family, is involved in regulating artemisinin biosynthesis. The physical interaction of AaAPK1 with AabZIP1 was confirmed by multiple assays, including yeast two-hybrid, bimolecular fluorescence complementation, and pull-down. AaAPK1, mainly expressed in flower buds and leaves, could be induced by ABA, drought, and NaCl treatments. Phos-tag mobility shift assays indicated that AaAPK1 phosphorylated both itself and AabZIP1. As a result, the phosphorylated AaAPK1 significantly enhanced the transactivational activity of AabZIP1 on the artemisinin biosynthesis genes. Substituting the Ser37 with Ala37 of AabZIP1 significantly suppressed its phosphorylation, which inhibited the transactivational activity of AabZIP1. Consistent overexpression of AaAPK1 significantly increased the production of artemisinin, as well as the expression levels of the artemisinin biosynthesis genes. Our study opens a window into the regulatory network underlying artemisinin biosynthesis at the post-translational level. Importantly, and for the first time, we provide evidence for why the kinase gene AaAPK1 is a key candidate for the metabolic engineering of artemisinin biosynthesis.
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正向调控青蒿素生物合成的 bHLH 转录因子 AabHLH1 的克隆和表征
影响因子：
4.9
作者：
Ji, Yunpeng;Xiao, Jingwei;Wang, Hong
通讯作者：
Wang, Hong
DOI：
10.1073/pnas.0505667103
发表时间：
2006-02-07
影响因子：
11.1
作者：
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通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
4.9
作者：
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影响因子：
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作者：
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拟南芥 RAV1 转录因子被 SnRK2 激酶磷酸化，在种子萌发和早期幼苗发育过程中调节 ABI3、ABI4 和 ABI5 的表达
影响因子：
7.2
作者：
Feng, Cui-Zhu;Chen, Yun;Chen, Yi-Fang
通讯作者：
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